Enzymatic and non-enzymatic antioxidants changes in precancerous and cancerous lesions of oral cavity
Journal Title: Medpulse International Journal of Biochemistry - Year 2018, Vol 5, Issue 2
Abstract
Background: Involvement of ROS in the development of tobacco related oral carcinogens is speculated. Little is known about the status of ROS scavengers or antioxidant enzymes in association with precancerous and cancerous conditions of oral cavity. In view of this, we investigated the parameters of antioxidants (enzymatic and non-enzymatic) in lymphocytes which are part of the body’s immune system and more sensitive to oxidative stress. Material and Methods: The study involved the analysis of various parameters using established standard methods. Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxide (GSH-Px), Glucose 6 phosphate dehydrogenase (G6PD) activities and Reduced Glutathione(GSH) were determined in the blood lymphocytes of patients with precancerous lesions (OSMF and Leukoplakia) and biopsy proven well differentiated Squamous cell carcinoma, enrolled in Oral Radiology department of Nair Dental College and Hospital and from healthy age and sex matched control. Plasma levels of non-enzymatic antioxidants β- carotene, Retinol, α-tocopherol and vit C and plasma levels of TBARS expressed as MDA was determined to find the extent of lipid peroxidation. Results: Enzyme activity was represented as mean ± S.D. and the data was analysed statistically. Student –t was applied to study the comparison of mean enzyme activity of healthy control group with patient groups and correlation coefficient (r) were computed using SPSS software. A highly significant decrease in the levels of SOD, CAT and GSH-Px G6PD and GSH (P<0.001) was noted in cancerous group. In precancerous cases all the enzymes showed moderate to significant fall in the activities. A moderate to significant fall in the plasma levels of β- carotene, retinol, vit c and α-tocopherol was noticed in both the groups as compared to the controls. MDA level expressed significant rise (P<0.001) in precancerous and cancerous group as compared to control. Conclusion: The antioxidant enzyme activity was reduced and lipid peroxidation was increased in oral precancerous and cancerous group.
Authors and Affiliations
Sarita Basu, V Naga Guhan
Keywords
Related Articles
- EP ID EP272575
- DOI -
- Views 178
- Downloads 0
Prevalence of metabolic syndrome and its components among young adults at a tertiary care hospital in Puducherry
Background: Metabolic syndrome refers to a group of risk factors that increase the risk for the development of type 2 diabetes mellitus and cardiovascular diseases. The prevalence of metabolic syndrome is increasing worl...
Correlation of 24-hour urinary protein, urinary protein: Creatinine ratio and HBA1c in type 2 diabetes with and without nephropathy
Background: The leading cause of morbidity and premature mortality in diabetics is nephropathy. closely associated with renal involvement in diabetes. Objectives: To estimate serum levels of HbA1c, 24 ratio in Type 2 dia...
Serum Ceruloplasmin/Albumin ratio in HIV patients with anti retroviral therapy
Background: HIV is a life long illness and requires regular monitoring and treatment as per the clinical, immunological and viral status of HIV patients. The various simple cost effective prognostic markers need to be ex...
A study of effect of insulin therapy on the endothelial dysfunction in the type II diabetic patients at tertiary health care centre
Background: Diabetes is an independent risk factor for cardiovascular disease1. Hyperglycemia2 and insulin resistance Aims and Objectives: To Study effect of insulin therapy on the endothelial dysfunction in the type II...
Serum lipid profile in oral submucous fibrosis - A case control study
Background: Oral Sub Mucous Fibrosis (OSMF) is a chronic, debilitating disease characterised by juxtaepithelial fibrosis of the oral cavity. It is a precancerous condition a generalized pathological state of the oral muc...