Toxicity of Thymol on the Ultra-Scanning Structure of Skin and Digestive Gland Proteins of the Two Slugs ‘Limax maximus and Lehmannia marginata’
Journal Title: The Egyptian Journal of Hospital Medicine - Year 2018, Vol 71, Issue 6
Abstract
<span>Background</span><span>: thymol is a natural derivative of herb thyme and extracted from </span><span>Thymus vulgaris</span><span>.<br /> </span><span>Aim of the work</span><span>: the present investigation was carried out to study the side effects of thymol on the skin and protein content in the digestive gland of both slugs: </span><span>Limax maximus </span><span>and </span><span>Lehmannia marginata</span><span>.<br /> </span><span>Materials and Methods: </span><span>the slugs are classified into three groups: the first one served as a control, the second and third groups fed on LC</span><span>50 </span><span>and LC</span><span>90 </span><span>of thymol for 48 hours.<br /> </span><span>Results: </span><span>the histological and scanning electron microscopic (SEM) observations of skin of LC</span><span>90</span><span>-treated slugs exhibited erosion of the epithelial cells, hypoplasia of the connective tissues with increased mucus secretion. Moreover, the different protein bands of the control and treated slugs with LC</span><span>50 </span><span>and LC</span><span>90 </span><span>of thymol were demonstrated by SDS-PAGE technique. A total number of 37 different protein bands were ranged from 5.181 to 84.375 kDa.<br /> </span><span>Conclusion: </span><span>the present study supported the use of thymol as a molluscicide agent on the skin and digestive gland proteins of both slugs (</span><span>Limax maximus </span><span>and </span><span>Lehmannia marginata</span><span>) that offers a safe alternative to other more persistent chemical pesticides that can be dispersed in runoff and produce subsequent contamination. </span>
Authors and Affiliations
Eman Helal
Keywords
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