Use of mutagenesis to improve stem thickness of sugarcane somaclone IBP 89-169
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 2
Abstract
The present work was realized with the aim to improve the somaclon IBP 89-169 selected as highly resistant to the smut of the sugarcane (Ustilago scitaminea Syd) and procedent of the variety Ja 60-5, but imposibilited to the production for to be too much thin. Calli were irradiated with 15 Gy of radiations Gamma obtaining 10 000 individuals, these plants were inoculated with a conidial suspension and were planted to field conditions, the selection was developed twelve months later. One evaluation in five zones of the population was realized before to selection to study the enviroment effect in the variability expresed, this evaluation showed a quite small effect. Twelve individuals with superior values to the 20.0 mm of diemeter of the stem were selected; in the clonal study were obtained three somaclons (IBP 01-14, IBP 02-51 e IBP 36-39) with better tolerance to (U. scitamineae Syd) and girth of the stem than the variety to improve (Ja 60-5).
Authors and Affiliations
Leonardo García Rodríguez, Pedro Orellana, Lourdes R. García, Juan N. Pérez Ponce, Novisel Veitía, Idalmis Bermúdez-Caraballoso, Justo Clavero García, Carlos Romero
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