3D Oral Squamous Cell Carcinoma Microtissues Grown in Calcium Alginate Microbeads
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 13, Issue 4
Abstract
3D microtissue models, especially cancer microtissue model, are potentially applicable for new drug testing because 3D microtissue reveals more realistic drug response, characterizes the disease and mimics the tumor in human body. In this paper, we report the integration of oral squamous cell carcinoma (OSCC) cell line (ORL-48) into 3D microtissue after two weeks of microencapsulation in calcium alginate microbeads that were produced based on the flicking technique. The microtissues contained highly proliferative cells as indicated by Alamar blue assay. The viable microtissues formed were extracted from the calcium alginate shell by means of alginate lyase. As revealed by field emission scanning electron microscopy (FE-SEM), the extracted 3D microtissues were characterized by inhomogeneous microtissue surface but good cell integrity via self-secreted extracellular matrix proteins. DAPI staining showed the proliferative behavior of cells in multilayer structure. These microtissues were able to spread into 2D monolayer after being transferred to grow on petri dishes. The 3D cultured microtissues of ORL-48 could be potentially useful for cancer therapeutic drug assessment in-vitro.
Authors and Affiliations
Soon Chuan Wong, Chin Fhong Soon, Kian Sek Tee, Wai Yean Leong, Mohd Khairul Ahmad, Sok Ching Cheong
Keywords
Related Articles
- EP ID EP313339
- DOI 10.9734/ARRB/2017/33526
- Views 86
- Downloads 0
Optimization of Somatic Embryogenesis in Ferula assa-foetida L. (a Rare Medicinal Plant)
Experiments was carried out to examine the effect of abscisic acid (ABA), source of carbon and temperature on secondary somatic embryogenesis and somatic embryo germination of Ferula assa-foetida L. Cotyledonary somatic...
Parameters of Facial, Nasal, Maxillary, Mandibular and Oro-facial Heights of Adult Urhobos of Nigeria
The study was carried out to determine the mean values of facial, nasal, maxillary, mandibular and oro-facial heights of adult Urhobos: one of the ethnic groups in Nigeria and comparism with some other ethnic groups in N...
Molecular Genetic Markers for Assessing the Genetic Variation and Relationships in Lactuca Germplasm
The genus Lactuca L. belonging to the Asteraceae family, is widely distributed in different geographical and ecological areas. Lettuce and most of the other species of the genus Lactuca L. have been cultivated for their...
Molecules of Interest – Mangiferin – A Review
Mangiferin is a 1,3,6,7-tetrahydroxyxanthone-C2-β-D-glucoside that has been reported to occur naturally in several genera. It exhibits a wide array of biological activities and also in several cases is responsible for th...
Phytodiversity Assessment in Abandoned Solid Waste Dumpsites in Port Harcourt, Nigeria
Aim: Assessments of phytodiversity at two abandoned solid waste dumpsites in Port Harcourt, Nigeria. Study Design: A systematic sampling approach comprising three line-transects of 5 m and 10 m interval was used. Methodo...