4-Thio-uridylate (UD29) interferes with the function of protein -SH and inhibits HIV replication in vitro.
Journal Title: Pharmacological Reports - Year 2009, Vol 61, Issue 2
Abstract
In this short communication, it is shown that 4-thio-uridylate (s(4)UMP, designated as UD29) inhibits glyceraldehyde 3-phosphate dehydrogenase (GAPDH), suggesting that the enol-form of the thiolated nucleotide may interfere with the function of the essential -SH group in the active center of the enzyme. Since HIV entry requires thiol/disulfide exchange processes, this activity prompted us to study the anti-HIV activity of the nucleotide. Indeed, UD29 inhibited the replication of HIV-1(IIIB) in the MT-4 cell line and HIV-1(Ada-M) in peripheral blood mononuclear cells (PBMC). Furthermore, UD29 was not toxic in PBMCs in vitro or in mice when the compound was administered intravenously.
Authors and Affiliations
Zoltán Beck, Andrea Kis, Erika Berényi, Péter Kovács, László Fésüs, János Aradi
Keywords
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