A Biochemical and In Vitro Study on the Protective Effects of Quercetin on Lysosomal Glycohydrolases, Cathepsins and Adenosine Triphosphatases in Isoproterenol Induced Myocardial Infarcted Rats
Journal Title: Journal of Enzymology and Metabolism - Year 2017, Vol 3, Issue 1
Abstract
This study was aimed to evaluate the protective effects of quercetin on lysosomal glycohydrolases, cathepsins and membrane bound adenosine triphosphatases in isoproterenol induced myocardial infarcted rats. Male albino Wistar rats were pretreated with quercetin (10 mg/kg body weight) daily for a period of 7 days. After the pretreatment period, isoproterenol (100 mg/kg body weight) was injected subcutaneously into rats twice at an interval of 24 h to induce myocardial infarction. The activities of serum creatine kinase and lactate dehydrogenase were increased significantly (P<0.05) and the activities of lysosomal enzymes (β-glucuronidase, β-N-acetyl glucosaminidase, β-galactosidase, cathepsin-B and cathepsin-D) were increased significantly (P<0.05) in the serum and heart of isoproterenol induced myocardial infarcted rats. Isoproterenol also decreased the activities of β-glucuronidase and cathepsin-D in the lysosomal fraction and enhanced in the cytosolic fraction. The activity of sodium/potassium dependent adenosine triphosphatase was decreased significantly (P<0.05) and the activities of calcium and magnesium dependent adenosine triphosphatases were increased significantly (P<0.05) in isoproterenol induced myocardial infarcted rats. Pretreatment with quercetin (10 mg/kg body weight) showed significant (P<0.05) protective effects on cardiac marker enzymes, lysosomal glycohydrolases, cathepsins and adenosine triphosphatases in isoproterenol induced myocardial infarcted rats. The in vitro study confirmed the strong reducing property of quercetin. Thus, the results of our study reveal that quercetin minimizes isoproterenol induced myocardial damage by reinstating the activities of lysosomal enzymes and adenosine triphosphatases to near normal levels. The observed effects are due to the membrane stabilizing and reducing properties of quercetin.
Authors and Affiliations
Stanely Mainzen Prince Ponnian
Keywords
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