A Comparative Analytical Quality Evaluation Study between Two Methods for Blood Urea Nitrogen Estimation
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 6
Abstract
The present study was undertaken to compare the analytical quality of 1) Diacetyl Monoxime Method and 2) Berthelot’s Method of estimation of blood urea. The performance characteristics were experimentally analysed to evaluate the analytical range, percentage recovery, sensitivity, accuracy, precision and detection limit of the two test methods. Performance of the methods were judged using statistical techniques. In a rural locality, due to interrupted electric power supply and inadequate UPS system for backup power, it was felt necessary to introduce a reliable manual backup method for blood urea test.Comparative analysis based on performance characteristics criteria indicate which of the two methods is acceptable. In the present study, Diacetyl monoxime method shows better analytical recovery, analytical sensitivity, detection limit with good accuracy and precision, as compared to Berthelot’s method which is not acceptable.Although Diacetyl Monoxime method shows variation from that of CLIAstandards, it can be considered for use as a manual backup method, by stringent observance of specifiedstandard precautions during the analytical process, to minimize the errors and improve analytical quality. Good laboratory practices require individual laboratories to evaluate analytical quality of methods and apply routinely.
Authors and Affiliations
Vinay G. Patke, Gaurav S. Kansara
Keywords
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