A Fluorescence Microscopy Study of Seminal Fluid In Infertile Males Using Acridineorange Dye To Assess DNA Integrity Along With Semen Analysis
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 10
Abstract
According to World Health Organization(2000), infertility is the inability of the sexually unprotected, active couple to achieve pregnancy in one year of marriage . Present study is aimed to assess DNA damage in sperms using Acridine Orange fluorescence technique in infertile malesto correlate the results of DNA damage with other semen parameters and to select the healthy semen donors in sperm banks as part of treatment of infertility. Collaborating the findings from our study and the previous studies in the literature, it is concluded that fluorescence microscopy of seminal fluid using acridine orange is an easy test to perform and a relatively cheap method as compared to other methods which are expensive and require more machinery, equipment’s and skilled technical staff. Although easy to perform and relatively cheap, it does not loses its relevance in semen analysis in comparison to other tests.
Authors and Affiliations
Dr. Gaurav Pawar, Dr. Kuldip Kumar, Dr. Chanpreetkaur pawar, Dr. Manoj K. Sharma, Dr. Jaspinderpratap Singh, Dr. Harmohinder K Attri
Keywords
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- EP ID EP401593
- DOI 10.9790/0853-1710032532.
- Views 92
- Downloads 0
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