A Lectin with Larvicidal Potential from the Fresh Leaves of Agelanthus brunneus (Engl.) Van Tiegh Loranthaceae
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 13, Issue 3
Abstract
Aims: This study reports the physicochemical properties and larvicidal activities of a lectin isolated from the leaves of Agelanthus brunneus (Engl.) Van Tiegh with a view to exploring its potential application and usage. Methods: The fresh leaves were homogenized with PBS and partial purification of the lectin was carried out by gel filtration on Sephadex G-75; physicochemical and biological properties of the purified lectin were also determined. Results: The results revealed that lectin from A. brunneus leaves agglutinated non-specifically the red blood cells of the human ABO system as well as the rabbit erythrocytes and the haemagglutinating activity was inhibited by lactose. The lectin was stable over a broad temperature range (up to 70°C) and maximum activity was observed in the 3 - 7 pH range. EDTA had no inhibitory effect on its haemagglutinating activity. Lectin activity was found to be maximal when photosynthesis was at its peak. The lectin was found to be toxic on Culex quinquefasciatus (Say) with LC50 of 0.24 mg/ml. Conclusion: The findings of the present study clearly revealed that the lactose-specific lectin from A. brunneus was an effective larvicidal agent against C. quinquefasciatus mosquito larvae and could be developed as biological control agent.
Authors and Affiliations
Imoh I. Johnny, Adenike Kuku, Oludele O. Odekanyin, Simeon K. Adesina
Keywords
Related Articles
- EP ID EP342257
- DOI 10.9734/BJPR/2016/28577
- Views 117
- Downloads 0
Anxiolytic, Sedative and Hypothermic Effects of Aqueous Leaf Extract of Vernonia amygdalina Del. (Asteraceae) in Albino Mice
Aims: This study evaluated the anxiolytic, sedative and hypothermic effects of aqueous leaf extract of Vernonia amygdalina in Mice. Study Design: One-factor two control groups experimental design. Place and Duration of...
Formulation and Evaluation of Dexibuprofen Transdermal Films in Rabbits
Dexibuprofen is a practically water-insoluble nonsterodial anti-inflammatory drug. Following previous experience on enhancing the solubility of the drug, a mixed hydrotropic solubilization technique was here applied in o...
Anti-proliferative Effect of Chickpea Extract on Hela, MCF-7, Saos and Fibroblast Cell Lines
Background and Aim: The present study aimed to investigate the proliferation inhibitory effects of seed protein extract from chickpeas (Cicer arietinum L.) on cancer cell lines. Materials and Methods: The chickpea water...
Antimicrobial and Therapeutic Potentials of the Ethylacetate Fractions of Crude Methanolic Extract of Monodora myristica Seed
Aim: The study was designed to evaluate the antimicrobial properties and potential therapeutic applications of Monodora myristica seed. Study Design: Antimicrobial evaluation design. Place and Duration of Study: The stu...
Antimicrobial and Cytotoxic Activities and GC-MS Analysis of Phytocomponents of Methanolic Extract of Curculigo pilosa (Schum and Thonn) Engl. (Hypoxidaceae) Rhizomes
Objectives: To investigate the antimicrobial and cytotoxic activities of crude methanol extract of Curculigo pilosa (Schum and Thonn) Engl. (Hypoxidaceae) rhizomes and its solvent soluble fractions and to analyze the mos...