A Modified Genomic DNA Extraction Method from Leaves of Sunflower for PCR Based Analyzes
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 7, Issue 6
Abstract
Sunflower genomic DNA extraction for PCR (Polymerase Chain Reaction) analyses and similar molecular technics is a challenging issue because of high concentrations of secondary metabolites, i.e. polyphenolics, polysaccharides, tannins and other contaminants. Our modified (Cetyl Trimethyl Ammonium Bromide) CTAB protocol can be performed approximately in two hours and suitable for PCR analyses. It needed small amounts of plant leaves and good quality of sunflower genomic DNA can be isolated. Quantity and purity of extracted DNA were checked by spectrophotometry and agarose gel electrophoresis. This modified method takes less time, reliable and gives good quality of DNA even without using RNase can be used in various marker systems such as (Single Sequence Repeat) SSR and (Random Amplified Polymorphic DNA) RAPD.
Authors and Affiliations
Hossein Zeinalzadehtabrizi, Arash Hosseinpour, Murat Aydin, Kamil Haliloglu
Keywords
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