A new approach for development of vaccine against visceral leishmaniasis: Lipophosphoglycan and polyacrylic acid conjugates
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2017, Vol 10, Issue 9
Abstract
Objective: To determine the antileishmanial vaccine effectiveness of lipophosphoglycan (LPG) and polyacrylic acids (PAA) conjugates on in vivo mice models. Methods: LPG molecule was isolated and purified from large-scale Leishmania donovani parasite culture. Protection efficacies of LPG alone, in combination with Freund's adjuvant, in a physical mixture and in conjugate (consisting of various LPG concentrations) with PAA, were comparatively determined by various techniques, such as cultivation with the micro-culture method, assessment of in vitro infection rates of peritoneal macrophages, determination of parasite load in liver with Leishman-Donovan Units, and detection of cytokine responses. Results: Obtained results demonstrated that the highest vaccine-mediated immune protection was provided by LPG-PAA conjugate due to all parameters investigated. According to the Leishman-Donovan Units results, the sharpest decline in parasite load was seen with a ratio of 81.17% when 35 mg LPG containing conjugate was applied. This value was 44.93% for the control group immunized only with LPG. Moreover, decreases in parasite load were 53.37%, 55.2% and 65.8% for the groups immunized with 10 mg LPG containing LPG-PAA conjugate, a physical mixture of the LPG–PAA, and a mixture of LPG + Freund's adjuvant, respectively. Furthermore, cytokine results supported that Th1 mediated protection occurred when mice were immunized with LPG-PAA conjugate. Conclusions: It has been demonstrated in this study that conjugate of LPG and PAA has an antileishmanial vaccine effect against visceral leishmaniasis. In this respect, the present study may lead to new vaccine approaches based on high immunogenic LPG molecule and adjuvant polymers in fighting against Leishmania infection.
Authors and Affiliations
Adil M. Allahverdiyev
Keywords
Related Articles
- EP ID EP250539
- DOI 10.1016/j.apjtm.2017.09.001
- Views 135
- Downloads 0
Comparative genome analysis between Southeast Asian and South American Zika viruses
Objective: To understand the cause for the differences between potentially mild Southeast Asian and the more pathogenic ZIKV in South America. Methods: A comparative genomic analysis was performed to determine putative c...
The first laboratory-confirmed SFTS case from the Xinjiang Uygur Autonomous Region importing to Hainan International Tourism Island
Severe fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by a tick-borne phlebovirus, which was reported to be continuously epidemic in Japan, Korea and in 23 provinces of China since 2010...
Clinical study inpatient-reported outcomes after binocular implantation of aspheric intraocular lens of different negative spherical aberrations
Objective: To compare patient-reported outcomes after implantation of the ZA9003 intraocular lens (IOLs), or the MCX11 ASP IOLs or the spherical IOLs (HQ-201HEP). Methods: Prospective nonrandomized controlled trial was u...
Evaluating the effect of Myrtus communis on programmed cell death in hydatid cyst protoscolices
Objective: To evaluate the possible involvement of programmed cell death strategy in hydatid cyst protoscolices following treatment with Myrtus communis (M. communis) as an herbal medicine. Methods: Protoscolices were as...
Effect and mechanism of Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia through connexin43 (cx43)
Objective: To explore the effect and mechanism of angiotensin II receptor blockers – Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia. Methods: Rats with embryonic cardiomyocytes-H9c2...