A RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION RIBAVIRIN IN TABLETS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 2
Abstract
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of Ribavirin (200mg) in tablets.Methods: The optimized method uses a reverse phase column, Enable Make KromasilC18 (250 X 4.6 mm; 5μ), a mobile phase of phosphate buffer (pH 4.2): acetonitrile in the proportion of 85:15 v/v, flow rate of 1.0 ml/min and a detection wavelength of 215 nm using a PDA detector.Results: The developed method resulted in Ribavirin eluting at 2.606 min. Ribavirin exhibited linear in the range 25-150μg/ml. The precision is exemplified by the relative standard deviation of 0.4%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) was found to be 0.24ng/ml and 0.73ng/ml respectively.Conclusion: An accurate, precise and linear RP-HPLC method was developed and validated for the quantitative estimation of Ribavirin in VIRAZIDE (200mg) tablets as per ICH guidelines and hence it can be used for the routine analysis in various pharmaceutical industries.Â
Authors and Affiliations
G. Raveendra Babu, A. Lakshmana Rao, J. Venkateswara Rao
Keywords
Related Articles
- EP ID EP579033
- DOI -
- Views 83
- Downloads 0
ROLE OF VICIA FABA FRUIT EXTRACT AGAINST CYTOTOXICITY INDUCED BY ACETAMINOPHEN IN PRIMARY CULTURED RAT HEPATOCYTES
Objective: In the present study, Vicia faba fruit extract was screened for their hepatoprotective activity against cytotoxicity induced by acetaminophen in primary cultured rat hepatocytes.Methods: Vicia faba fruit, seed...
ANTIMICROBIAL ACTIVITY OF NATURAL CHLOROPHYLLIN FROM ENDANGERED MEDICINAL PLANT MIMOSA PUDICA L.
Objective: Chlorophyllin (CHL) belongs to a group of compounds, porphyrins that contain a chelated metal ion in the center of the molecule. The objective of this present study was to extract the Chlorophyllin from Mimosa...
IMPACT OF PROLONGED REDUCED-PRESSURE CONDITION PRIOR TO PRECURSOR LABELING ON THE LABELING EFFICIENCY OF F-18 FLUOROCHOLINE SYNTHESIS
Objective: The goal of this preliminary work was to observe the impact of the prolonged reduced-pressure condition prior to labeling stage on the F-18 Fluorocholine labeling yield at the end of synthesis.Methods: At this...
IN VITRO DPPH RADICAL SCAVENGING AND ANTI-BACTERIAL ACTIVITY OF OMAN’S CYMBOPOGON
Objective: The objective of this work is to evaluate antioxidant and anti-microbial activity of methanolic extract of Omani Cymbopogan schoenanthus.Methods: Antibacterial activity of mehanolic extract of Cymbopogan was e...
MINING SINGLE NUCLEOTIDE POLYMORPHISM FROM PUBLICLY AVAILABLE ESTS OF BREAD WHEAT (TRITICUM AESTIVUM L.)
Objective: The present study was undertaken to discover Single Nucleotide Polymorphisms (SNPs) in bread wheat with reference to leaf rust disease.Methods: Next Generation Sequencing platform sequencing by Oligonucleotide...