A REVIEW ON TIME CORRELATED SINGLE PHOTON COUNTING
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 4
Abstract
The phenomenon of molecular fluorescence emission contains both spectral and time information it is former which has traditionally been more usually associated with routine analytical applications of fluorescence spectroscopy. In contrast, since the early seventies, the most important strides in fluorescence spectroscopy have been by and large concerned with the development and application of new time resolved techniques. Applications of these now span not only in the traditional areas of photochemistry but also research in polymers, membranes, liquid crystals, low-dimensional structures, solar energy, and semiconductors, to name but a few. Such diverse areas are linked by their common instrumentation needs, which have greatly benefited over the past decade from quite radical progress in optoelectronics and on-line data analysis. There is now widespread recognition of the advantages of studying and using fluorescence in the time domain. Quite simply, fluorescence measurements in the time domain (fluorometry) posses a much greater information content about the rates and hence kinetics of intra and intermolecular processes than is afforded by the wavelength spectroscopy of the energy domain (fluorimetry). A simple analogy to the greater detail shown by time domain as compared to steady state fluorescence measurements is that of motion pictures in comparision to still photographs. The rising importance of fluorescence measurements in the time domain has been reflected during the past decade in several texts devoted exclusively to this topic. Nevertheless, the field has not stood still, and, if anything, the rate of innovation has probably increased over the past few years
Authors and Affiliations
Vineela P*, , Vivek Sagar , Pavani B , Ajitha A, , Uma Maheshwara Rao. V
Keywords
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