A Study on Expression of Vascular Endothelial Growth Factor in Colorectal Malignancies and its Correlation withVarious Clinicopathological Parameters
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 1
Abstract
ABSTRACT Introduction: Tumours require neovascularisation for growth and metastasis. Vascular Endothelial Growth Factor (VEGF) is an important regulator of tumour angiogenesis and is associated with metastasis and poor prognosis. Aim: To assess the expression of VEGF in colorectal cancer by Immunohistochemistry (IHC), to compare it with the clinicopathological parameters and to determine the prognostic significance. Materials and Methods: We received 147 cases of resected specimens of colorectal carcinomas for histopathological examination in Institute of Pathology, Madras Medical College, Chennai, Tamil Nadu, India, during the period between July 2013 to July 2015. Relevant clinical details of the patients undergoing surgery for colorectal malignancy, regarding age, gender, procedure done were collected. Four μm thick sections were taken from formalin fixed paraffin embedded tissue blocks and stained with haematoxylin and eosin. Among them, 50 random cases were selected for immunohistochemical studies. Results: The median age at presentation was 60 years. Maximum number of cases occurred in the age group of 51-60 years. There was a male preponderance with 85 cases (57.8%). Left sided tumours were more common (104 cases, 70.7%). About 53.7% cases had tumours less than 5 cm in greatest dimension. Most common gross appearance was ulceroproliferative (92 cases, 62.6%). Most common histopathological subtype was infiltrating adenocarcinoma (123 cases, 83.7%). Most common grade was moderately differentiated (95 cases, 64.6%). Fifty three cases (36.1%) belonged to stage C2, 66 cases (44.9%) had lymph node involvement, 74 cases (50.3%) had lymphatic invasion, 67 cases (45.6%) had vascular invasion and resected margins were free in 143 (97.3%) cases; 4 cases (2.7%) had tumour infiltration in their resected margins. Among the 50 cases on which IHC was done, 9 (18%), 20 (40%) and 21 (42%) cases had 1+, 2+ and 3+ levels of VEGF expression respectively. A total of 21 cases (42%) had strong cytoplasmic staining for VEGF and 15 cases (30%) and 14 cases (28%) showed moderate and weak cytoplasmic VEGF positivity respectively. Statistically significant association was found between VEGF expression in colorectal carcinoma and factors like presence of lymphatic invasion, vascular invasion, lymph node metastasis and stage of the tumour. Conclusion: VEGF expression in colorectal malignancies seems to be an independent prognostic marker of tumour behavior and may be used to identify patients with unfavourable clinical outcome.
Authors and Affiliations
K AShwini, RAJAGOPALAN PADMAVATHI
Keywords
Related Articles
- EP ID EP506145
- DOI 10.7860/JCDR/2018/28992.11033
- Views 62
- Downloads 0
Expression of Oct4 Protein in Astrocytic Tumours-Histopathologic and Immunohistochemical Study
ABSTRACT Introduction: The majority of gliomas in adults are resistant to therapy. Therapy resistance has been attributed to the presence of cancer stem cells (CSCs). Oct4 is a transcription factor required for maintaini...
A Prospective Study on the Prevalence of Pulmonary Artery Hypertension in Thyroid Dysfunction
Introduction: Though rare, thyroid dysfunction is known to cause Pulmonary Artery Hypertension (PAH) which is also known to be reversible by restoration of euthyroid state. Hence, timely diagnosis and institution of medi...
A Comparative Study of Body Composition and Sleep Habits Between Male and Female Students
ABSTRACT Introduction: Elevated body composition is associated with many health hazards and it is also strongly associated with sleep habits of individuals. Aim: To compare body composition and sleep habits between male...
Investigation of ABO Blood Groups in Periodontal Status and its Effect on Level of Response to Nonsurgical Periodontal Treatment
ABSTRACT Introduction: ABO blood groups have been associated with several systemic diseases. Various studies have investigated the relationship between ABO blood groups and periodontal diseases. Aim: The purposes of this...
Spontaneous Intracranial Hypotension
Images in Medicine