A Validated High Performance Liquid Chromatography Method for the Determination of Saxagliptin and Metformin in Bulk and Pharmaceutical Dosage Form, a Stability Indicating Study
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
A Stability indicating reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous estimation of Saxagliptin (SAX) and Metformin (MET) in bulk and pharmaceutical dosage form. The separation was carried on kromasil- C18 column (4.5 x 250 mm; 5 µm) column with mobile phase consisting of 50mM sodium dihydrogen phosphate buffer (pH adjusted to 2.7 using orthophosphoric acid): methanol in the ratio of 80:20 v/v with a flow rate of 0.9 ml/min and PDA detection at 242nm. The linearity was found to be in range of 0.5-3 µg/mL (R2 = 0.9995) and 50-300 µg/mL (R2 = 0.9995) for SAX and MET respectively. The method has shown good, consistent recoveries for SAX 98.39-101.53% and MET 100.46 -101.59% respectively. The method was found to be accurate, precise, specific, robust and linear for the determination of SAX and MET in bulk and pharmaceutical dosage form.
Authors and Affiliations
Ramesh J, Dr. Senthil Kumar N
Keywords
Related Articles
- EP ID EP386791
- DOI 10.9790/3008-11060392100
- Views 66
- Downloads 0
Résultats DeLa Trabeculoplastie Sélective sur La Baisse Pressionnelle Dans Le GPAOResults of Selective Trabeculoplasty on Pressure Reduction in POAG
Pour vérifier les résultats tonométriques de SLT dans le traitement du glaucome primaire à angle ouvert une étude rétrospective a été réalisée dans notre unité glaucome. 116yeux de 58 patients ont bénéficié de la procédu...
The Inhibitory Activity Of Medicinal And Aromatic Plants Used In Folklore Medicine In Jazan Region Of Kingdom Saudi Arabia
Treatment of various human disorders with folkloric medicine has been in practice for centuries. This study was conducted on twenty medicinal and aromatic plants found in Jazan region of the Kingdom of Saudi Arabia. The...
Biosynthesis of Iron Oxide Nanoparticles using Aqueous Extract of Convolvulus Pluricaulis Plant, its Characterization and Acute Oral Toxicity Studies
The synthesis of nanoparticles by green approach has been of great interest because of its ecofriendly and non-toxic potential in biomedical applications. The present study was aimed to synthesize Convolvulus pluricaulis...
Comparative In Vitro Equivalence Evaluation of Some Loratadine Generic Tablets Marketed in Bangladesh
Loratadine is a potent, rapidly effective and long-acting non-sedative, histamine H1 receptor antagonist, with anti-allergic properties. The purpose of this research work was to evaluate the pharmaceutical equivalent of...
Role of Mecp2 Gene in Neuro Developmental Disorders
The increasing emergence of Complex Neurological Disorders (NDDs) necessitates genotypic approaches to characterize therelevant phenotypes. The analysis of complex NDDs have shown that the genetics is fundamental to such...