A validated RP HPLC method for simultaneous estimation of lamivudine and tenofovir disoproxil fumarate
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2016, Vol 4, Issue 2
Abstract
A precise, simple, selective and accurate RP-HPLC method was developed and validated for rapid assay of Lamivudine and Tenofovir fumarate in pharmaceutical dosage form. Lamivudine and Tenofovir disoproxil fumarate are anti-retro viral drugs and both are nucleotide reverse transcriptase inhibitors. Isocratic elution is carried out at the flow rate of 1.0ml/min. The chromatographic separation was performed using Inertsil ODS3V column (4.6*250mm), 5µm particle size. Mobile phase contains phosphate buffer and acetonitrile 55:45(v/v) at pH 3 and the detection wavelength was at 264nm. Linearity was observed in concentration range of 70-170µg/ml for Lamivudine and 60-140 µg/ml Tenofovir disoproxol fumarate. The observed retention time for Lamivudine is 2.893min and 5.420min for Tenofovir disoproxil fumarate. The proposed method was validated based on ICH guidelines. The suggested method could be efficiently used for the assay of Lamivudine and Tenofovir dosoproxil fumarate in tablet dosage forms.
Authors and Affiliations
L. Mohan Krishna, Srinivasa Rao Konijeti, B. Prabhakar Reddy, K. Ashok Kumar
Keywords
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- EP ID EP33369
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