ABO ambiguous blood types in voluntary blood donors from Putian, Fujian Province: a serology and molecular biology study
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 6
Abstract
Objective To investigate the serological and molecular biology characteristics of ambiguous blood types among voluntary blood donors in Putian, and to file records for relevant donors for future reference in case of their own transfusion needs or emergency donations to others. Methods A total of 68 593 blood samples from voluntary blood donors in Putian Central Blood Station between January 1, 2019 and August 31, 2023 were collected and tested for blood types using serological methods. ABO gene (including promoters, enhancers and seven exons as well as their flanking sequences and intron 6) and FUT1 gene testing were performed on ambiguous blood types.3D models were constructed using Chimira and PyMOL software to predict the impact of gene mutations on enzyme structure. Results A total of 16 ABO subtypes were identified by serological methods, with the highest detection rate as the para-Bombay phenotype (0.73 per 10 000), followed by the cisAB phenotype (0.44 per 10 000). Gene analysis revealed 12 cases with known mutations (4 cases of FUT1.01N.06/FUT1.01N.06, 1 case of FUT1 01W.08/FUT1.01N.06, 2 cases of A2.08/B.01, 1 case of BA.02/O.01.01, 1 case of A3.07/O.01.01, 3 cases of cisAB.01/B.01), and key mutations were not found in 4 cases (2 cases of A1.02/B.01, 2 cases of A1.02/O.01.02). 3D molecular model analysis revealed that both A3.07 and FUT1.01W.08 allele can lead to a decrease in activity of the corresponding glycosyltransferases, resulting in the emergence of subtypes. Conclusion The most common phenotype causing discrepancies in ABO blood type testing among voluntary blood donors from Putian is the para-Bombay phenotype, with the most common allele being FUT1.01N.06.
Authors and Affiliations
Tiehui LIN, Xueling ZHENG, Xiaohong CAI, Hang LEI
Keywords
Related Articles
- EP ID EP742783
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.06.008
- Views 24
- Downloads 0
Hemolytic transfusion reaction analysis and domestic literature review
Objective To investigate the causes of hemolytic transfusion reaction (HTR), identify clinical symptoms and severity, explore clinical detection methods, so as to provide reference for safe and rational blood use. Method...
Evaluation of the efficacy and safety of human coagulation factor Ⅷ in the treatment of hemophilia A patients
Objective To evaluate the efficacy and safety of human coagulation factor Ⅷ developed by Shenzhen Weiguang Biological products Co, Ltd in the treatment of patients with hemophilia A. Methods A prospective, multi-center,...
Characteristics of whole blood donors from 26 blood stations before and after the outbreak of COVID-19:a multicenter study
Objective To analyze the basic characteristics of whole blood donors from blood stations before and after the outbreak of COVID-19. Methods After excluding invalid data, data related to the basic characteristics of whole...
Expert consensus on recipient blood type test and blood component transfusion after ABO-incompatibility hematopoietic stem cell transplantation
At present, ABO-incompatibility hematopoietic stem cell transplantation (ABOi-HSCT) accounts for 30%~50% of the total HSCT, and is no longer a major obstacle to HSCT. Transfusion therapy is an important treatment for HSC...
Comparison of clinical efficacy of fibrinogen concentrate and cryoprecipitate on severe postpartum hemorrhage in puerpera with placenta accreta
Objective To explore the therapeutic effect of fibrinogen concentrate and cryoprecipitate on severe postpartum hemorrhage in puerpera with placenta accreta under the condition of transfusion of suspended red blood cells...