Abstract

Algal biomass a potential renewable feedstock for 3rd generation biofuel only if the production costs are substantially reduced avoiding added stress on water resources. The waste water generated from various sources has substantial amounts of nutrients load are useful for algae growing. Selection of proper waste water and its ready access in large volume round the year remain a big concern. While wastewater as such cannot be used for algal culture it needed dilution to right concentration and robust algal strains either screened or acclimatized. This research finding reports the three steps of acclimatization process of Chlamydomonas sp. BTA 4152 for growing in rubber wastewater (RW). In the first step 13.3 x 104 cells/ml of cells were grown in 20, 40, 60, 80, 100% RW recorded highest growth (83.1 x 104 cells/ml) with net increase of 5.2 folds in 20% RW after 2 weeks. The same cells cultured in 20,40,60,80 and 100% RW showed highest growth (1024 x 104 cells/ml) with net increase of 2.22 fold in 40% RW in 2 weeks. Finally the acclimatized cells in 40% RW cultured in 40, 60, 80 and 100% RW highest growth (1450 x104cells/ml) with net increase of 39.28 folds and 504mg/l dry biomass in 5 weeks.

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