Acetyl and Butyrylcholinesterase Inhibiting Constituent from Morinda lucida Benth (Rubiaceae)
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 6, Issue 5
Abstract
Aims: This study identified the cholinesterase inhibitory principle in Morinda lucida, one of the plants used in Nigerian ethnomedicine as memory enhancer. Study Design: In vitro anti cholinesterase assay and spectroscopic analysis of isolated compound. Place and Duration of Study: Department of Pharmacognosy, Obafemi Awolowo University, Nigeria between 2010 and 2012. Methodology: Activity directed phytochemical analysis using Thin Layer Chromatography (TLC) bioautographic assay and repeated Vacuum Liquid Chromatography followed by Preparative Thin Layer Chromatography (PTLC) on silica gel was used to isolate one compound from the most active fraction of the plant. Results: Spectroscopic analysis (H1 and 13C NMR) and comparison with literature data identified this compound as phytol (3, 7, 11, 15-tetra methyl- 2-hexadecen-1-ol), with an IC50 of 12.93 µg/ml acetyl cholinesterase (AChE) and 24.90 µg/mlbutyryl cholinesterase (BuChE). Conclusion: Isolation of compound with cholinesterase inhibitory activity has to some extent validated the ethnomedical use of the plant as memory enhancer and has provided new information on the chemistry of the plant.
Authors and Affiliations
Taiwo Olayemi Elufioye, Efere Obuotor, Joseph Morounfolu Agbedahunsi, Saburi Adejimi Adesanya
Keywords
Related Articles
- EP ID EP344471
- DOI 10.9734/BJPR/2015/16491
- Views 94
- Downloads 0
Natural Sources of Taxol
Taxol® is the most successful anticancer agent developed in the last 50 years. The alkaloid taxol and other taxoids were at first isolated from T. brevifolia. Because of the small yield that threatened existence of the n...
Development and Validation of Bio Analytical Method for Estimation of Bortezomib in k3 EDTA Human Plasma Using HPLC-ESI-MS/MS and Its Application to a Bioequivalence & CME Studies
Purpose: To develop a highly selective, reproducible & precise rugged bio analytical method for estimation of Bortezomib (BTZ), “A Protease Inhibitor” in human plasma by validating the developed method in accordance to U...
Tracheostomy and Innominate Artery Bleeding: A Late Complication
Tracheostomised patients are at risk of several complications. The complications of tracheostomy include bleeding, stenosis, pneumothorax, tracheo-arterial fistulas and malacia. In article described the case of severe co...
Quantitative Structure-activity Relationships (QSAR) and Docking Studies on Pyrimidine Derivatives for Antitubercular Activity against M. tuberculosis H37Rv
Aim: QSAR techniques and docking increase the probability of success and reduce time and coast in drug discovery process. The study presents QSAR investigation on 20 pyrimidine derivatives for antitubercular activity aga...
Preparation, Characterization and Optimization of Poloxamer Solid Dispersions of a Poorly Water Soluble Drug Aprepitant
Aim: The aim of the present investigation was to enhance the dissolution rate of a poorly water soluble drug, aprepitant, by preparation of solid dispersion with hydrophilic carrier, poloxamer 188, using solvent evaporat...