Activity Guided Fractionation with Antimicrobial Evaluation of Pergularia tomentosa L. (Asclepiadacea) Whole Plant
Journal Title: Microbiology Research Journal International - Year 2015, Vol 8, Issue 5
Abstract
This research work aimed to present the activity guided fractionation with antimicrobial evaluation from both crude extract and various fractions obtained from Pergularia tomentosa L. whole plant. The P. tomentosa L. whole plant was extracted with 95% aqueous ethanol; fractionated into acidic, basic, polar and nonpolar fractions. All fractions with the crude extract were screened for both antimicrobial and minimum inhibitory concentration (MIC) potentialities. For crude extract, all concentrations (1.5, 0.75, 0.35, & 0.168 mg/ml) indicated marginal antibacterial activity with range of 17, 20, 14 mm zone of inhibition for S. aureus, E. coli and C. albicans. While, both basic fraction showed highest activity against E. coli and C. albicans at 15 mm & 15 mm; along with acidic and methanolic fraction haven large spectrum against S. aureus, E. coli and C. albicans at 13, 12, 12 mm. Moreover hexane did not showed antimicrobial activity for both S. aureus and C. albicans except for E. coli which showed activity at 12 mm. The study clearly indicated that basic fraction showed highest antimicrobial activity for selected micro-organisms with lower minimum inhibitory concentration which ranges from 18.75 µg/ml to 150 µg/ml. Followed by wider spectrum of antimicrobial activity for acidic and methanolic fraction against all tested organisms with minimum inhibitory concentration from 75 µg/ml to 150 µg/ml; while 300 µg/ml (MIC) stand for hexane fraction. Thus, P. tomentosa L. particularly, the basic fraction (alkaloid) and, both acidic and neutral fractions will be centered areas for further scientific research findings in isolating an active antimicrobial component therein.
Authors and Affiliations
Rufai Yakubu, Fatima Musa, Aminu Lukman
Keywords
Related Articles
- EP ID EP354347
- DOI 10.9734/BMRJ/2015/17027
- Views 78
- Downloads 0
Prevalence of Waterborne Diseases and Microbial Assessment of Drinking Water Quality in Ado-Ekiti and Its Environs, Southwestern, Nigeria
Aims: This study aimed to determine the prevalence of waterborne disease and assess the microbial quality of drinking water sources in Ado-Ekiti and its environs. Study Design: Cross-sectional, descriptive type of study....
Morphology and Ecology of Freshwater-blooming Durinskia baltica (Dinophyceae: Peridiniales) in Xochimilco, Mexico
Framework and Aims: A dinoflagellate identified as Durinskia baltica has caused early spring blooms since 2007 in a eutrophic freshwater channel system influenced by agriculture and livestock in central Mexico. The goals...
Seroepidemiology of Incidentally Detected Asymptomatic HBsAg Positive Subjects from Southern State of India – A One Year Study
Introduction: Hepatitis B virus infection is assuming a silent epidemic phase in India. Majority of the chronically infected are asymptomatic and unaware of their status. This pool of individuals called; incidentally det...
Evaluating Efficacy of Biosurfactants from Bacterial Isolates in Conferring Protection against Rhizoctonia and Sclerotium Infection in Wheat and Peanut Plants
Aims: To isolate biosurfactant producers from natural habitat and to test the antimicrobial activity of the extracted biosurfactant against fungal plant pathogens. Study Design: The present study was designed to achieve...
Retro-respective Evaluation of New Fixed Dose Combination of Antibiotics in Management of Severe Skin and Soft Tissue Infections – A Comparative Pharmacoeconomic Study
Study Background: Skin and soft tissue infections (SSTIs) are the second most common infection encountered in hospitals. Present study aims to comparatively analyze efficacy of new fixed dose combination (FDC) with teico...