Activity of Catambra Extracts against Meloidogyne spp.
Journal Title: Journal of Experimental Agriculture International - Year 2015, Vol 5, Issue 3
Abstract
Aims: In this study we tested four Catambra commercial formulates (hydroalcoholic extracts) used as insecticides in Italy, against Meloidogyne incognita and Meloidogyne javanica. Catambra plant (Ambrogios, Catalpa) a European patent belonging to Ambrogio srl, rich in an iridoid glycoside catalpol, is used to date as a mosquito deterrent, but no data exist on its biological activity against soil parasites as Meloidogyne sp. Catalpa species demonstrate various biological properties of pharmaceutical and agronomical interest, mainly due to their contents in glycosides. Place and Duration of Study: All paralysis bioassays were performed in Pesticide Science Laboratory, of Aristotle University Thessaloniki, Greece, while chemical analysis in Department of Life and Environmental Sciences, University of Cagliari. Materials and Methods: The activity was tested in paralysis bioassays with freshly hatched second-stage juveniles (J2) immersed in test solutions for four time periods (12, 24, 48 and 96 hours). Catalpol contents of the extracts were measured by means of HPLC/MS and its activity was also tested against J2. The effect of exhaustive evaporation of hydroalcoholic extracts prior use in bioassays was also studied for nematicidal activity. Results: Out of the four extracts tested without being evaporated first, two extracts (C-2 and C-4) exhibited activity and the EC50/48h on M. incognita were calculated at 640 and 728 μg/ml. On the other hand, M. javanica was proven sensitive only to the C-4 extract and the activity had an EC50/48h value at 500 μg/ml. When extracts were exhaustively evaporated before use, only the C-4 paralysed J2 and the EC50/48h values were 728 and 500 μg/ml against M. incognita and M. javanica, respectively. Interestingly, catalpol was not found to be nematicidal at the concentration range of 100 to 1000 μg/ml. The nematicidal activity of the extracts was therefore not correlated with their content in catalpol. This was confirmed by the chemical composition study, since the C-4 extract, exhibiting activity against both species, was of low contents in catalpol (21 μg/ml), and the C-3 extract that had the highest contents in catalpol (358 μg/ml) did not paralyse J2. Conclusion: Catambra nematicidal activity is not based in its contents in catalpol, and further studies are conducted to delineate for activity among other constituent components.
Authors and Affiliations
Nikoletta G. Ntalli, Eleni Nasiou, Chrysostomos Oplos, Federico Ferrari, Urania Menkissoglu- Spiroudi
Keywords
Related Articles
- EP ID EP354067
- DOI 10.9734/AJEA/2015/12211
- Views 116
- Downloads 0
Evaluation and Agronomic Potential of Androgenic Pepper Genotypes Derived from Piran (Capsicum annuum L. cv. Piran)
Aims: This study was to evaluate and estimate the agronomic potential of two androgenic genotypes via important plant, fruit and production traits compared to the mother genotype Piran. Place and Duration of Study: Strum...
Efficacy of Selected Insecticide and Biopesticide Combinations against Major Insect Pests of Late Maturing Pigeonpea [Cajanus cajan (L.) Millsp.]
Aim: To evaluate the comparative efficacy of different chemical insecticide and biopesticide combinations against tur pod fly [Melanagromyza obtusa (Malloch)], gram pod borer [Helicoverpa armigera (Hübner)] and tur pod b...
Determinants of Access to Credit by Agribusiness Operators in the Kumasi Metropolis, Ghana
Aim: The study was conducted to analyze the factors that influence access to credit by Agribusiness operators in the Kumasi Metropolis of the Ashanti Region of Ghana. Study Design: The study used a multi-stage sampling t...
Profitability of Yam-Maize-Soybean Enterprise among Resource Poor Farmers Using Herbicide for Weed Control in the Northern Guinea Savanna
This study examined the profitability of herbicide weed control in yam-maize-soybean enterprise, factors influencing it, as well as the problems encountered by the farmers using this weed control measures. The study was...
Forage Intake and Wastage by Ewes in Pea/Hay Barley Swath Grazing and Bale Feeding Systems
Harvested feed costs, particularly during the winter, are traditionally the highest input associated with a ruminant livestock operation. Although swath grazing has been practiced for over 100 years and literature exists...