Adenovirus-mediated overexpression of gamma interferon in murine bone marrow-derived dendritic cells affects their viability and activity
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 0
Abstract
Objective: To explore the effects of induction of Gamma-interferon(IFN-γ) production by adenoviral vectors (AdVs) on dendritic cells (DCs) viability and activity. Methods: AdVs were propagated in human embryonic kidney 293 cell lines and DCs were derived from mouse bone marrow using GM-CSF-IL-4-enriched media. The DCs were infected by either AdVs-green fluorescent protein or AdVs-IFN-γ during a 48 h culture. Phenotypic and functional characteristics of AdV-infected DCs were measured by flowcytometry-based methods. Results: Our results displayed that AdVs-IFN-γ could significantly induce DCs necrosis. Furthermore, AdVs-IFN-γ-infected DCs efficiently expressed the CD86 molecules. Conclusions: According to our finding, AdV-IFN-γ is able to affect murine bone marrowderived DC viability and activity.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP230475
- DOI 10.1016/S2222-1808(14)60470-9
- Views 69
- Downloads 0
First determination of impact and outcome indicators following indoor residual spraying (IRS) with deltamethrin in a new focus of anthroponotic cutaneous leishmaniasis (ACL) in Iran
Objective: To determine the impact and outcome indicators following residual spraying with deltamethrin in new focus of cutaneous leishmaniasis during 2010-2011. Methods: Deltamethrin WP 5% was applied at 25 mg a.i/m2.in...
Soil transmitted helminths in animals – how is it possible for human transmission?
Objective: To determine the current prevalence of soil transmitted helminths (STH) infections among cats and dogs in an animal shelter. Methods: A total of 442 animal's faecal samples were collected from the selected ani...
Prevalence of intestinal parasitic infections among schoolchildren in Phitsanulok Province, Northern Thailand
Objective: To determine the prevalence of intestinal parasites in children from six schools located in Bang Rakam and Mueang Districts, Phitsanulok Province, Northern Thailand. Methods: The study sites were the following...
Pharmacognostic study and anti - inflammatory activity of Phyllanthus reticulatus Poir. fruit
Objective: To study pharmacognostic evaluation and anti - inflammatory activity of Phyllanthus reticulatus Poir. (P.reticulatus) fruit. Methods: The fruit sample was studied by macroscopical, microscopical and other WHO...
Comparison of parasite infection degree in cattle ( B o s sp.) using faecal egg counting method in two East Java regions, Lamongan and Gresik
Objective: To investigate the type of parasites in cattle in Lamongan and Gresik, East Java, Indonesia and their infection degree using faecal egg counting. Methods: Parasite egg analysis was performed using Whitlock met...