Agarose cell block technique as a complementary method in the diagnosis of fungal osteomyelitis in a dog

Journal Title: Open Veterinary Journal - Year 2012, Vol 2, Issue 4

Abstract

A 7-year-old Labrador Retriever female dog presenting left forelimb lameness for one day was admitted to the Veterinary Hospital (UNESP-Botucatu) for clinical evaluation. Several tests, including blood and image analysis, microbiological culture and cytology of lytic areas of affected bone were made in order to establish a diagnosis. Serum biochemical profile revealed increased levels of liver enzymes, plasma globulin, creatine kinase (CK) and calcium. Hemogram revealed anemia and leukocytosis; left humerus image analysis revealed an osteolytic lesion and cytology revealed a suppurative periostitis. Differential diagnosis was a nonspecific infectious inflammatory process or osteosarcoma. Since it was not possible to achieve a definitive diagnosis and there was a highly suspicious for an infectious agent, an agarose cell block of the bone marrow fine-needle aspiration was made. The cytological examination of cell block presented similar findings as described previously. However, additional stains including periodic acid-Schiff (PAS) were positive for fungal hyphae, which rendered a diagnosis of fungal osteomyelitis due to Aspergillus spp. This case report illustrates an uncommon cause of osteomyelitis for breed that was diagnosed by an underused method in veterinary medicine.

Authors and Affiliations

D. S. Zanoni| Laboratory of Investigative and Comparative Pathology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista – UNESP. Botucatu, Brazil, F. Grandi| Laboratory of Investigative and Comparative Pathology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista – UNESP. Botucatu, Brazil, Department of Pathology, Botucatu Medical School, Univ. Estadual Paulista – UNESP. Botucatu, Brazil, D. Q. Cagnini| Laboratory of Investigative and Comparative Pathology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista – UNESP. Botucatu, Brazil, S. M. G. Bosco| Department of Microbiology and Immunology, Biosciences Institute, Univ. Estadual Paulista – UNEP, Botucatu, Brazil, N. S. Rocha| Laboratory of Investigative and Comparative Pathology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista – UNESP. Botucatu, Brazil, Department of Pathology, Botucatu Medical School, Univ. Estadual Paulista – UNESP. Botucatu, Brazil

Keywords

Related Articles

Occurrence and surgical repair of third degree perineal lacerations in adult female camels (Camelus dromedarius) by one-stage (Goetz) technique

Retrospective analysis of third degree perineal lacerations in 7 female camels (6-17 yrs of age) that were surgically corrected by one stage repair (Goetz technique) is presented. Majority (3/7) of the camels was primip...

A three years retrospective study on the nature and cause of ocular dermoids in cross-bred calves

Nature and cause of ocular dermoids were investigated by field studies, pedigree analysis, clinical examination and light microscopy. It was determined that ocular dermoids in cross-bred calves are genetically-transmitt...

Effect of melatonin on maturation capacity and fertilization of Nili-Ravi buffalo (Bubalus bubalis) oocytes

This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspi...

Exophthalmos associated to orbital zygomatic mucocele and complex maxillary malformation in a puppy

A case of exophthalmos due to zygomatic mucocele in a puppy with ipsilateral segmental maxillary atrophy is reported. A 7-month-old, mixed breed, male dog suffered the sudden-onset of unilateral painful exophthalmos and...

Ultrasound and multidetector computed tomography of mandibular salivary gland adenocarcinoma in two dogs

Malignant tumors of the salivary glands are rare in dogs, with adenocarcinoma being the most represented. Parotid and mandibular glands are most commonly affected in dogs. Because of local invasivity and high metastatic...

Download PDF file
  • EP ID EP9256
  • DOI -
  • Views 427
  • Downloads 26

How To Cite

D. S. Zanoni, F. Grandi, D. Q. Cagnini, S. M. G. Bosco, N. S. Rocha (2012). Agarose cell block technique as a complementary method in the diagnosis of fungal osteomyelitis in a dog. Open Veterinary Journal, 2(4), 19-22. https://europub.co.uk./articles/-A-9256