Alpha-bungarotoxin (α-Bgtx) and beta-bungarotoxin (β-Bgtx) binding activities in human cadaver brain
Journal Title: Archives of Medical Science - Year 2007, Vol 3, Issue 2
Abstract
Introduction: Using 125Iα-Bgtx as a ligand, postsynaptic cholinergic nicotinic receptors has been identified in species. Presynaptic receptors can be defined as receptors at or near the nerve terminal that can positively or negatively modulate transmitter release, that directly or indirectly influence the probability of an action potential resulting in exocytosis. These receptors are identified by utilizing b-Bgtx. Our study involves the identification of these receptors in different regions of brain. Material and methods: The activity of postsynaptic AChR and presynaptic membrane receptor in membrane preparation and triton extract of muscle and brain tissue was determined by virtue of its high affinity for a and b-Bgtx by rapid filtration method in case of membrane preparations and ammonium sulphate precipitation method in triton extracts i.e; solubilized receptor. Results: Specific α-Bgtx binding as fmoles/mg tissue was observed in occipital lobe (6.1±0.1) parietal lobe (6±0.6), temporal lobe (5.2±1.4), cerebral cortex (6.2±0.3), hippocampus(5.6±0.2), hypothalamus (4.3±0.4) and brain stem (4.8±0.2). No specific binding of α-Bgtx was found in cerebellum and frontal lobe. β-Bgtx binding activity was present in all the above regions of brain and was in range of 4.2-4.9 fmoles/mg tissue. Presynaptic receptors also shows immunoreactivity with myasthenic sera. Conclusions: In addition to muscle, β-Bgtx binding proteins are present in each regions of the brain, while as a-Bgtx binding proteins are absent in some regions. β-Bgtx binding sites could play an important role in the pathogenesis of myasthenia gravis.
Authors and Affiliations
Suhail Rasool, Bansi Jailkhani, Mohd Irshad, Madhuri Behari, Vinay Goel
Keywords
Related Articles
- EP ID EP133966
- DOI -
- Views 133
- Downloads 0
Cervical spondylosis: a literature review with attention to the African population
Adenosine and neutrophil CD11b/CD62L and oxidative burst in stable ischemic heart disease
Objectives: The immune response to endogenous as well exogenous stimuli is modified by various factors including drugs. Adenosine is an endogenous nucleotide involved in cellular energy metabolism. Adenosine receptors ma...
Preprocedural fibrinogen levels and MACE after percutaneous coronary intervention
Introduction: Fibrinogen is a risk factor for cardiovascular disease and is related to the severity of coronary atherosclerosis. According to many studies elevated fibrinogen levels in patients with coronary angioplast...
Episodes of severe menorrhagia at menarche in a girl with von Willebrand disease
We describe a case of a 14-year-old girl with von Willebrand disease (vWD) and episodes of severe menorrhagia at menarche, necessitating hospitalization and requiring blood transfusions and administration of purified von...
Predictors of atrial fibrillation in patients following isolated surgical revascularization. A metaanalysis of 9 studies with 28 786 patients
Introduction: Atrial fibrillation (AF) is one of the most common complication following cardiac surgery AF significantly increases the length of intensive care unit and hospitalization stay and worsens the early and long...