Alteration in CatSper1 and 2 genes expression, sperm parameters and testis histology in varicocelized rats
Journal Title: International Journal of Reproductive BioMedicine - Year 2018, Vol 16, Issue 3
Abstract
Background: CatSper gene, a member of cation channel sperm family, has an essential role in sperm motility and male fertility. Following varicocele, sperm parameters especially sperm movement decreases. For this reason, we hypothesized that CatSper gene expression might be reduced after varicocele induction in an animal model. Objective: The aim of this study was to evaluate the expression of CatSper 1 and 2 genes, sperm parameters and testis histology following varicocele induction. Materials and Methods: A total of 30 Wistar male rats were randomly divided into three following groups (n=10/ each): control, sham, and varicocele group. Experimental varicocele was induced by partial ligation of the left renal vein. The epididymal sperm parameters, CatSper1 and 2 genes expression, and testes histology were studied two months after varicocele induction. Results: Our results revealed that motility (32.73±16.14%), morphology (48.80±17%) and viability (31.23±9.82%) of sperms significantly reduced following varicocele induction. In addition, we showed a significant decrease in the number of spermatogonia (43.63±5.31) and seminiferous tubules diameters (190.51±19.23 mm) in experimental varicocele rats. The level of CatSper1 and 2 genes expression evaluated using real-time polymerase chain reaction was significantly downregulated 2 months after varicocele induction. Conclusion: Our data indicated that experimental varicocele has deleterious effects on sperm parameters, testis structure as well as the expression of CatSper 1 and 2 genes.
Authors and Affiliations
Maryam Zohour Soleimani, Farideh Jalali Mashayekhi, Morteza Mousavi Hasanzade
Keywords
Related Articles
- EP ID EP283352
- DOI 10.29252/ijrm.16.3.183
- Views 106
- Downloads 0
Assessing the efficacy of vaginal hyoscine butyl bromide on cervical ripening prior to intrauterine procedures: A double-blinded clinical trial
Background: Unripe cervix prevents entering the endometrial cavity during intrauterine procedures. Mechanical dilatation of cervical canal might cause undesirable complications. Objective: To investigate the substitute o...
The relationship of the psychological coping and adjustment strategies of infertile women with the success of assisted reproductive technology
Background: The success of assisted reproductive techniques plays a very important role in the quality of life of infertile couples and decreases the negative behavior states of infertility. Objective: This study aimed a...
Investigating the effects of Citrullus colocynthis pulp on oxidative stress in testes and epididymis in streptozotocin-induced diabetic male rats
Background: Diabetes mellitus is one of the most common metabolic diseases in humans, affecting 100 million people around the world. Objective: Investigating the effects of Citrullus colocynthis pulp on oxidant and antio...
Oxidative stress induced sperm DNA damage, a possible reason for male infertility
Background: Sperm DNA damage is an important factor in the etiology of male infertility. Objective: The aim of the study was to evaluate the association of oxidative stress induced sperm DNA damage with the pathogenesis...
Subchronic effects of different doses of Zinc oxide nanoparticle on reproductive organs of female rats: An experimental study
Background: Zinc performs many biochemical and physiological functions; however, toxicological studies demonstrate that Nano-zinc oxide has harmful effects on human health and environmental species in high concentrations...