Alteration of the Methylation Status of Urokinase Plasminogen Activator (uPA) is involved in Proliferation and Invasion of Nasopharyngeal Cancer Cells
Journal Title: Archives of Otolaryngology and Rhinology - Year 2015, Vol 1, Issue 1
Abstract
The extracellular matrix degradation is the most important step in the process of tumor cell metastasis. Urokinase-type plasminogen activator (uPA) can catalyzes the conversion of the inactive zymogen plasminogen to the active broad-spectrum plasmin, which degrades a number of matrix proteins and also activates other proteases, including some matrix metalloproteinases. In this study, we evaluated that effect of methylation status of urokinase plasminogen activator (uPA) on invasion and metastasis of nasopharyngeal cancer cells. Methylation status of urokinase plasminogen activator (uPA) was detected by Methylation-specific PCR in the nasopharyngeal carcinoma cells. Reverse transcription-PCR was used to detect the expression of uPA mRNA in the nasopharyngeal carcinoma cell. Invasive and proliferation capacity of CNE2 cell was detected by using Boyden chamber Matrigel invasion assay and Cell proliferation assay after MBD2 knockdown. Our data demonstrated that uPA gene promoter hypomethylation is related to strong uPA mRNA expression in nasopharyngeal cancer cell, uPA mRNA expression was significantly reduced after MBD2 knockdown in the CNE2 cells, the proliferation and invasion capacity of CNE2 cells was significantly inhibited after MBD2 knockdown. Our data suggest that reversal of uPA gene hypomethylation will become a novel therapeutic approach for blocking nasopharyngeal cancer progression and metastatic.
Authors and Affiliations
Yang Ju-Hong, Lu Chun-Sheng, Zhang Song
Keywords
Related Articles
- EP ID EP350579
- DOI 10.17352/2455-1759.000004
- Views 119
- Downloads 0
Temporal Bone Trauma: To Pull Down the Wall of Incommunicableness
In the past decades, treatment of the temporal bone traumas (TBTs) was undeniably improved by advances in neuroradiological and in neuro-oto-surgical fields. However, these advances did not systematically enhance the qua...
Alteration of the Methylation Status of Urokinase Plasminogen Activator (uPA) is involved in Proliferation and Invasion of Nasopharyngeal Cancer Cells
The extracellular matrix degradation is the most important step in the process of tumor cell metastasis. Urokinase-type plasminogen activator (uPA) can catalyzes the conversion of the inactive zymogen plasminogen to the...
Influence of Cleft lip and Palate on Characteristics of Hearing Loss
Objectives: To find out and evaluate and compare hearing threshold of the cleft-side vs. non-cleft side in children with unilateral cleft lip and palate (UCLP), to find out if the severity of the morphological and functi...
Trans Tracheal Approach to the Oesophagus: Case Report
Hypopharyngeal squamous cell carcinoma (SCC) is a rare form of malignancy and treatment approach is most commonly with chemo-radiotherapy or total pharyngolaryngoesophagectomy. We report a case of hypopharyngeal SCC mana...
An extraordinary case of a hungry man: an unusual foreign body - A Case Report
This case reports a rare case of accidental metal spoon swallowed by a normal adult which was successfully removed without complication under local anesthesia using a McGill forceps via laryngoscope. Aerodigestive foreig...