An improved protocol for adipose tissue-derived stem cell isolation: implications for treatments of bone disorders
Journal Title: European Journal of Medical Technologies - Year 2014, Vol 2, Issue 1
Abstract
Adipose tissue-derived stem cells (ASC) have been shown to possess similar characteristics to bone marrow-derived mesenchymal stem cells (BM-MSC), with extensive proliferative potential and multilineage differentiation ability. They have also been found to be less immunogenic than BM-MSC, thus representing an excellent potential tool in regenerative medicine. Most of the protocols for ASC isolation utilize crude collagenase to digest fatty tissue. However, large discrepancies between isolation methods have resulted in diversity in cell yield and functional properties. Thus, to meet good manufacturing standards necessary for clinical use, there is a need to improve methods of ASC isolation. The aims of the current study were firstly to establish a novel improved protocol for ASC isolation making use of liberases. Secondly, to compare liberase-derived ASC to BM-MSC, in terms of surface antigen and gene expression profiles, proliferation potential and ability to differentiate into osteocytes. We showed that digestion of adipose tissue with liberases appeared to be superior to collagenase digestion, since significantly higher numbers of fibroblast colony forming units (CFU-F) were obtained. Liberase-3-derived ASC and BM-MSC both expressed similar surface antigens (CD105, CD73, CD90 and CD166) and were negative for hematopoietic cells markers (CD14, CD45 and HLA-DR). In addition, both liberase-3-derived ASC and BM-MSC differentiated into osteocytes. However, ASC showed significantly higher proliferative potential.
Authors and Affiliations
Sylwia Bobis-Wozowicz, Sophia Millington-Ward, Artur Zawisz, Tomasz Adamus, Paweł Konieczny, Marcin Majka
Keywords
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