AN IMPROVED RP-HPLC METHOD FOR THE QUANTITATIVE DETERMINATION AND VALIDATION OF LEVETIRACETAM IN BULK AND PHARMACEUTICAL FORMULATION
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2015, Vol 6, Issue 8
Abstract
The present study depicts the development of a validated RP-HPLC method for the determination of the Levetiracetam in pharmaceutical tablet dosage form. RP-HPLC method was developed by utilizing Welchrom C18 Column (250 x 4.6 mm, 5µ), Shimadzu LC-20AT Prominence Liquid Chromatograph. The mobile phase composed of 10 mM Phosphate buffer: acetonitrile (50:50 v/v) (pH-3.0, adjusted with triethylamine). The flow rate was set to 1.0 ml/min with the responses measured at 243.2 nm using Shimadzu SPD-20A Prominence UV-Visible detector. The retention time of Levetiracetam was found to be 3.030 minutes. Linearity was established for Levetiracetam in the range of 10-50µg/mlwith correlation coefficient 0.9998. The LOD and the LOQ for Levetiracetam found to be 0.468 μg/ml and 1.421 μg/ml respectively. The amount of Levetiracetam present in the formulation was found to be 99.56 %. None of the excipients interfered with the analyte of interest. Considering all the results of validation parameters simplicity of the method and the cost effectiveness of the overall procedure, it is possible to conclude that the developed method can be suitable for the regular quality control determination of Levetiracetam in bulk as well as pharmaceutical dosage form.
Authors and Affiliations
Ravisankar P, Niharika A, Babu Srinivasa
Keywords
Related Articles
- EP ID EP158935
- DOI 10.7897/2230-8407.068106
- Views 97
- Downloads 0
SIMULTANEOUS ESTIMATION AND VALIDATION OF PARACETAMOL AND DOMPERIDONE IN BULK AND TABLET DOSAGE FORM BY USING DIFFERENT SPECTOPHOTOETRIC METHOD
Three simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of Paracetamol and Domperidone in a binary mixture. In Method I absorbance were measured at 258 nm an...
TEST OF ANTIOXIDANT ACTIVITY LEAVES OF SCAEVOLA TACCADA (GAERTN.) ROXB. USING DPPH (1, 1-DIPHENYL-2-PICRYLHYDRAZYL)
Free radicals can caused damage cell and tissue which lead to various disease then needed antioxidant as radicals scavenging. The objective of this study was to determine antioxidants activity leaves of Scaevola taccada...
PREPARATION AND EVALUATION OF TIMOLOL MALEATE MUCOADHESIVE BUCCAL TABLETS
The present investigation was concerned with preparation and evaluation of mucoadhesive buccal tablets containing antihypertensive drug, Timolol maleate to circumvent the first pass effect and to improve its bioavailabil...
EVALUATION OF ANALGESIC POTENTIAL OF KIGELIA PINNATA LEAF EXTRACT IN WISTAR RATS
The main aim of the present study is to evaluate the analgesic activity of leaf extract of Kigelia pinnata on wistar rats. Analgesic activity of the leaf extract of Kigelia pinnata at a dose of 200 mg/kg & 400 mg/kg...
A REVIEW ON PHARMACOLOGICAL ACTIVITY OF SYZYGIUM CUMINI EXTRACTS USING DIFFERENT SOLVENT AND THEIR EFFECTIVE DOSES
Plants and their extracts have immense potential for the management and treatment of disease. The phyto-medicines used for treatment are not only cheap and affordable but are also purportedly safe as hyper sensitive reac...