ANALYSIS OF 4-HYDROXYCYCLOPHOSPHAMIDE IN CANCER PATIENTS PLASMA FOR THERAPEUTIC DRUG MONITORING OF CYCLOPHOSPHAMIDE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 9
Abstract
Objective: To quantify 4-hydroxycyclophosphamide in cancer patients’ plasma for therapeutic drug monitoring of cyclophosphamide.Methods: The blood was collected at 0.5 and 1 h after administration of chemotherapy. Prior to analysis, 4-OHCP in plasma was derivatized with semicarbazide HCl, then was extracted using 4 ml ethyl acetate and finally was determined by Ultra High-Performance Liquid Chromatography–tandem mass spectrometry. Chromatographic separation was conducted using waters acquity BEH C18 column (1.7 μm; 50 mm x 2.1 mm). The mobile phase consisted of formic acid 0.1% and methanol (50: 50, v/v), column temperature 30 °C and flow rate of 0.3 ml/min. Mass detection was performed on waters xevo TQD equipped with an electrospray ionization (ESI) source at positive ion mode in the multiple reaction monitoring (MRM). Cyclophosphamide was detected at m/z 260.968>139.978, 4-hydroxycyclophosphamide-semicarbazide at m/z 338.011>224.97, and hexamethylphosphoramide as internal standard at m/z 180.17>92.08.Results: The method was linear in the range of 5–1000 ng/ml for cyclophosphamide and also for 4-hydroxycyclophosphamide. The results showed that the level of 4-OHCP in 39 cancer patients was in the range of 5.02 ng/ml to 832.44 ng/ml.Conclusion: 4-hydroxycyclophosphamide was detected on 39 patient samples with the lowest level of 5.40ng/ml and the highest level was 832.44 ng/ml. This can be a parameter that the regiment of cyclophosphamide was effective.
Authors and Affiliations
Yahdiana Harahap, Christian Samuel, Rizka Andalusia, Nadia Farhanah Syafhan
Keywords
Related Articles
- EP ID EP576033
- DOI 10.22159/ijpps.2016v8i9.12918
- Views 65
- Downloads 0
STATISTICAL OPTIMIZATION OF MEDIUM COMPONENTS BY PLACKETT BURMAN DESIGN AND RESPONSE SURFACE METHODOLOGY FOR ENHANCED ANTIOXIDANT ACTIVITY BY XYLARIA FEEJEENSIS HMJAU22039
Objective: To enhance the antioxidant potential of Xylaria feejeensis by statistical optimization. The components of potato dextrose yeast extract (PDYEB) medium: potato infusion, dextrose and yeast extract were investig...
A SURVEY OF DRUG-RELATED-PROBLEMS AMONG PATIENTS TREATED FOR ALLERGY SYMPTOMS IN COMMUNITY PHARMACIES AT NEGERI SEMBILAN, MALAYSIA
Objectives: Patients treated for allergy symptoms are at risk of acquiring drug-related problems (DRPs), as they require various types and forms of medications. Inappropriate therapeutic management of allergy symptoms co...
MULTIVARIATE ANALYSIS BETWEEN THE PHYTOCHEMICAL FEATURES AND ANTIOXIDANT PROPERTIES OF THE STEMS OF BAUHINIA GLABRA JACQ. (FABACEAE)
Objective: To provide information about the phytochemical features of the crude extract (CruE) and fractions of the forageBauhinia glabra Jacq., Fabaceae, and present themultivariate correlation between its metabolites a...
IMPACT OF METFORMIN AND SERRATIOPEPTIDASE IN OBESE PATIENTS WITH KNEE OSTEOARTHRITIS
Objective: Knee osteoarthritis is a common disabling chronic disease globally. Many pharmacological agents have been used efficiently in treatment of knee osteoarthritis. This study aims to evaluate metformin and serrati...
PRO-INFLAMMATORY CYTOKINES ELICIT INFLAMMATORY RESPONSE IN BLOOD LEUKOCYTES OF POST DIALYTIC CHRONIC RENAL PATIENTS THROUGH HEME OXYGENASE-1 ACTIVATION
Objective: Inflammation is common in patients with chronic renal failure and has been associated with the increased production of pro-inflammatory cytokines. The aim of the investigation is to assess the levels of the pr...