ANALYSIS OF DNA PLASMID COMPOSITION OF SALT TOLERANT STRAINS OF RH IZOSPHERE MICROORGANISMS
Journal Title: Мікробіологія і біотехнологія - Year 2015, Vol 0, Issue 4
Abstract
The aim. The object of researches was study and analysis of plasmids composition of salt tolerant bacteria Bacillus subtilis SKB- 309, Bacillus megaterium SKB-310 and Pseudomonas stutzeri SKB-308 which are the part of “Zamin-M” preparation. The microbial composition «Zamin-M» on the basis of high-active and stable to stressful environmental conditions bacterial strains is successfully applied in agricultural practice of Uzbekistan. Methods. Bacteria which were the objects of researches are isolated from middle-saline soils and crops rhizosphere and selected by step screening of the strains growing in presence of 3–5 and 7% NaCl in growth medium and identified by Bergey. Results. As a result of electrophoretic analysis of plasmid DNA of salt-tolerant microbial strains by Birnboim and Dolli method the plasmid DNA with dimensions 48.5, 30 and 13.3 kb (kilobase) at B. subtilis, 23.1 kb at B. megaterium and 55 kb at P. stutzeri SKB-308 have been revealed. It is supposed that these plasmids can keep the genes of bacterial resistance to adverse environmental conditions, including high content (7%) of Cl- toxic ions.
Authors and Affiliations
S. S. Murodova, K. D. Davranov
Keywords
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- EP ID EP395226
- DOI 10.18524/2307-4663.2015.4(32).57464
- Views 81
- Downloads 0
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