Analysis of Influenza A/Beijing/353/89 Virus Matrix Gene Sequence and Identification of Replaceable Amino Acids as Natural Mutations in M2 Protein
Journal Title: Microbiology Research Journal International - Year 2016, Vol 15, Issue 6
Abstract
The influenza virus A/Beijing/353/89 matrix (M), M1 and M2 genes were cloned and sequenced. The sequence identity rates (based on published data) of the first 34 influenza A viruses with M genes most phylogenetically related to A/Beijing/353/89 virus were in significant positive correlation among M, M1 and M2 genes and M1 protein but not M2 protein. The number of viruses with M2 protein sequences identical to influenza A/Beijing/353/89 virus was significantly higher than those with identical M2 gene and M1 protein sequences (P<0.01). These results suggest that natural variation of M2 protein did not occur entirely in random. Based on a statistical standard we have established and using the A/Beijing/353/89 M2 as reference protein, 35 replaceable amino acids were identified by analysis of 193 published influenza A virus M2 protein sequences. In contrast, it is more possible that those critical amino acids for virus survival and/or replication harbored in the 24 amino acids, which were not yet found to have ever been substituted.
Authors and Affiliations
Yongxin Zhang, Monica Zhang, Ying Wang, Xyanthine Gilmore, Innocent N. Mbawuike
Keywords
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- EP ID EP350797
- DOI 10.9734/BMRJ/2016/27447
- Views 76
- Downloads 0
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