Analysis of Transforming Growth Factor-Beta1 Gene Polymorphisms in Macedonian Patients with Chronic Periodontitis
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2009, Vol 2, Issue 1
Abstract
Background. The existing conflicting data in the literature about the significance of TGF-beta1 polymorphisms in periodontitis. Aim. To determine whether TGF-beta1 polymorphisms are associated with periodontitis in Macedonian population. Material and methods. The sample consisted of 301 healthy unrelated individuals, and 132 patients with periodontitis. All individuals were of Macedonian origin and nationality and residents of different regions of the Republic of Macedonia. DNA was isolated from peripheral blood leukocytes by the phenol-chlorophorm extraction method. Cytokine polymorphism genotyping was performed by PCR-SSP (Heidelberg kit). The population genetics analysis package (PyPop) was used for analysis of the data. Crude odds ratios, were calculated with 95% confidence interval. P<0.05 was considered statistically significant. Results. Significant negative (protective) association between the Macedonian patients with periodontitis and: 1) cytokine genotypes TGF-beta1 cdn 10/C:T and TGF-beta1 cdn 25/G:G; 2) cytokine haplotypes TGF-beta1/TG and cytokine haplotype zygotes TGF-beta1/CG:TG were found. Positive (susceptible) association was found with: 1) cytokine alleles TGF-beta1 cdn 10/C and TGF-beta1 cdn 25/C; 2) cytokine genotypes TGF-beta1 cdn 10/C:C and TGF-beta1 cdn 25/C:G; and 3) cytokine haplotypes TGF-beta1/CC and cytokine haplotype zygotes TGF-beta1/ CC:CG. Conclusion. It is concluded that polymorphisms of TGF-beta1 gene are associated with an increased risk of chronic periodontitis in Macedonian population.
Authors and Affiliations
Aneta Atanasovska-Stojanovska| Dental Clinical Center, Department of Oral Pathology and Periodontology, Faculty of Stomatology, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia and , Dejan Trajkov| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia, Mirjana Popovska| Dental Clinical Center, Department of Oral Pathology and Periodontology, Faculty of Stomatology, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia and , Mirko Spiroski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss. Kiril and Metodij”, Skopje, Republic of Macedonia
Keywords
Related Articles
- EP ID EP8656
- DOI 10.3889/MJMS.1857-5773.2009.0029
- Views 369
- Downloads 21
Zdravko Trojacanec. Prevention of Sports Injuries. Skopje: Trojacanec; 2009. 151 pages; ISBN 978-9989-3-2; price MKD 300
Field of medicine: Prevention medicine. Audience: Sports medicine specialists, general practitioners, students in medicine. Purpose: Every mental activity of an individual is closely related and dependable on their ph...
Keratoconus and Its Prevalence in Macedonia
Aim. This is an epidemiological study which shows the prevalence and demographic distribution of keratoconus, and indicates the risk factors in aetiology of keratoconus in Macedonia. Material and methods. In this retro...
MICA Polymorphism, Association with Diseases and the Role of Anti-MICA Antibodies in Organ and Stem Cell Transplantation
Major histocompatibility complex class 1 chain-related genes (MIC) are part of the non-classical MHC genes located on the short arm of chromosome 6. MICA comprises of approximately 11 kb DNA and encodes polypeptide of...
Osteoskeletal Manifestation of Scurvy in a Male Infant (Case Report)
Scurvy, a disease of dietary deficiency of vitamin C, was uncommon in the last century due to improved nutrition and health care. However, in the last decade it was increasingly reported in adult population. In a perio...
Value of Ultrasound Machine Settings Optimization for Better Diagnosis of Focal Liver Lesions
Background. Focal liver lesions and metastasis can be very hard to localize. For best imaging, customizing of several settings of the machine are necessary: brightness, contrast, probe frequency, focusing, dynamic rang...