Analytical method development and validation for the estimation of Olmesartan medoxomil by RP-UPLC in bulk and pharmaceutical dosage forms.
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
A simple rapid, accurate, precise and reproducible validated reverse phase UPLC method was developed for the determination of Olmesartan medoxomil in bulk and pharmaceutical dosage forms. The quantification was carried out using Waters acquity UPLC BEH C18 (100 X 2.1 mm, 1.7 µm) column run in Isocratic way using pH 3.4 Buffer: Acetonitrile (60:40% v/v)] and a detection wavelength of 250nm, and injection volume of 4µL, with a flow rate of 0.3mL/min. The retention times of Olmesartan medoxomil was found to be 3.418. The method was validated in terms of linearity, precision, accuracy, LOD, LOQ and robustness in accordance with ICH guidelines. The linearity ranges of the proposed method lies between 0.080 mg/mL to 0.120 mg/mL, which is equivalent to 10% to 150% and with correlation coefficient of r2=0.9999.The assay of the proposed method was found to be 98.79%. The recovery studies were also carried out and mean % Recovery was found to be 100.5%. The % RSD from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Olmesartan medoxomil in bulk and in Pharmaceutical dosage form.
Authors and Affiliations
Farhana Pattan, Haritha Pavani, Chandana N, Karimulla M
Phytochemical evaluation of Cassia tora, Caesalpinia bonducela, Tecoma capensis
In the present study, an attempt was made to investigate phytochemical evaluation of Cassia tora, Caesalpinia bonducela, Tecoma capnensis. The crude drug powder extracts of the leaves of the above plants were taken for...
Preclinical evaluation of antispermatogenic activity of Curcuma amada on cell phone radiation induced infertility in rats
To study the ability of the electromagnetic signals emitted by cellular mobile telephony to affect the biological function of living organisms and mobile radiation exposures are changes in the structure and functional...
Effect of soaking pits on the quality of Ground water
Ground water purity is very important in human’s health. The present paper has shown the effect of soaking pits on the ground water. Fluoride is commonly present in the ground water. But due to soaking pits the quality...
Phytochemical, physicochemical and pharmacological profile of the selected plant Curcuma longa linn
In ethno medicinal practices, the traditional healers use the genus Curcuma for the treatment of various ailments Turmeric is a spice derived from the rhizomes of Curcuma longa, which is a member of the ginger family (...
Formulation and evaluation of biodegradable Ondansetron hydrochloride implant
Implantable drug delivery systems are used to deliver drugs for longer period of time, beneath the skin. These drug delivery systems are implanted subcutaneously and drug is supposed to be released in sustained manner...