Anthelmintic Activity of Hygrophila difformis Blume
Journal Title: Journal of Buffalo Science - Year 2012, Vol 1, Issue 1
Abstract
This study evaluated the anthelmintic activity of the petroleum ether, benzene, chloroform, ethanol extracts of aerial parts of Hygrophila difformis (Family: Acanthaceae). The Pheretima posthuma were used for all the experimental protocol due to their anatomical and physiological resemblance with the intestinal roundworms of ruminants and human beings. The worms were treated with the extract solutions containing three different concentrations (10, 25 and 30 mg/ml), for each extracts supplemented with 1% gum acacia in normal saline at 37 ± 1°C. Albendazole at a dose of 10 mg/ml was used as reference. The effectiveness of the extracts was judged on the basis of the loss of spontaneous movement and/or death of the worms. Qualitative phytochemical analysis of different extracts of the plant was carried out following the standard procedure. All extracts at different dose levels effectively paralyzed and killed the worm in dose-dependent fashion (p < 0.001). Maximum anthelmintic activity was found with benzene extract. Cardiac glycosides, tannins, steroids, flavonoids and saponins were found positive in phytochemical analysis. The present study established the anthelmintic activity of H. difformis and can be utilized for the ruminants.
Authors and Affiliations
Krishanu Samanta, Emdad Hossain and Dilip Kumar Pal
Keywords
Related Articles
- EP ID EP33523
- DOI http://dx.doi.org/10.6000/1927-520X.2012.01.01.07
- Views 433
- Downloads 0
Outbreak of Fatal Subacute Bubaline Fasciolosis in Wayanad, Kerala, India
Present communication deals with a fatal outbreak of subacute fasciolosis in male buffalo calves in Wayanad district. During first week of February 2012, six out of 25 male buffalo calves aged 6-9 months, owned by a far...
Metagenomic Analysis of Uterine Microbiota in Postpartum Normal and Endometritic Water Buffaloes (Bubalus bubalis)
In Indian subcontinent the water buffalo (Bubalus bubalis) is one of the important livestock animals. As in cows, postpartum infection like endometritis in dairy buffaloes is major cause for the economic loss in the dai...
Estimation of Body Weight and Body Surface Area in Swamp Buffaloes using Visual Image Analysis
The three dimensional computerized visual image analysis was performed to evaluate the body weight (BW) and body surface area (BSA) in swamp buffaloes. Nineteen swamp buffaloes were measured the conformation by linear...
Biochemical and Fatty Acids Composition of Water Buffalo (Bubalus Bubalis) Follicular Fluid
Aim of this study was to characterize the biochemical and fatty acids composition of follicular fluid collected from follicles of different sizes and in different phases of ovarian cycle in water buffalo farmed in Italy...
Haematological Profiles of Lactating Nili-Ravi Buffaloes Under Heat Stress-Alleviated Conditions During Sub-tropical Summer Season
The experiment was conducted using forty two Nili-Ravi lactating buffaloes of Central Institute for Research on Buffaloes, Regional Station-Bir Dosanjh, Nabha, Patiala, Punjab to investigate the effect of heat stress-al...