Anti-angiogenic Rather than Pro-angiogenic and Wound Healing-Promoting Effects of Lantana camara L. (Verbenaceae) in a Zebra Fish Model of Tissue Regeneration and in Cultured Human Umbilical Vein Endothelial Cells
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 9, Issue 4
Abstract
Aims: Plant-derived substances are extensively used for promoting wound healing in various traditional medicinal systems. In this study, extracts from such plants were evaluated for such properties using an animal model of tissue repair and angiogenesis and a cell culture model of proliferation, migration, and angiogenesis. Place and Duration of the Study: The study was carried out for sixteen months at the Departments of Pharmacology and Physiology of the Faculty of Medical Sciences, Anton de Kom University, Paramaribo, Suriname. Design and Methods: Aqueous extracts from parts of Aloe vera, Cinnamomum cassia, Lantana camara, Momordica charantia, Psidium guajava, and Solanum melongena were evaluated for their capacity to stimulate the regeneration of the amputated caudal fin of wild-type AB zebra fish embryos; the development of sub-intestinal vessels of Tg(fli1a:EGFP)y1/+ zebra fish embryos; the closure of scratch-wound gaps in human umbilical vein endothelial cell (HUVEC) monolayers; and capillary-like structure formation by these cells in matrigel. The data obtained were compared to those found with untreated controls and considered statistically significantly different when P values < .05 (Student’s t test). Results: None of the plant extracts stimulated fin regeneration and sub-intestinal vessel formation in the fish embryos or HUVEC scratch-wound closure and capillary-like structure formation. However, the L. camara extract delayed the regrowth of the amputated fin and the formation of sub-intestinal vessels by approximately 30 (P =.004) and 50% (P = 0.1), respectively, and inhibited HUVEC scratch-wound closure and capillary-like structure formation by roughly 100 (P = .004) and up to about 50% (P = .04 and .05), respectively. The remaining plant extracts did not affect either of these phenomena. Conclusion: The L. camara extract exerted marked anti-angiogenic characteristics rather than pro-angiogenic and wound healing-promoting properties. This makes it a candidate for evaluation in diseases caused by excessive angiogenesis.
Authors and Affiliations
D. R. A. Mans, J. R. Toelsie, M. Djotaroeno, P. Friperson, J. Pawirodihardjo, I. Magali, R. C. Soekhoe, K. Oedairadjsingh, J. A. Hasrat, R. Bipat
Keywords
Related Articles
- EP ID EP350300
- DOI 10.9734/EJMP/2015/19330
- Views 138
- Downloads 0
Liquidambar formosana Hance: A Mini-review of Chemical Constituents and Pharmacology
Aims: Liquidambar formosana Hance is of great an ornamental and economic arbor. Its’ various organs, such as the gum, fruits, barks, and leaves have a great medicinal value and commonly used in the pharmaceutical, food a...
A 28- Day Oral Toxicity Study of Pseudocedrela kotschyi Methanol Extract in Sprague-Dawley Rats
Aims: To evaluate the safety profile of Pseudocedela kotschyi which is used in traditional medicine for the treatment of epilepsy, malaria, diarrhoea and pains, a 28 day sub chronic toxicity study was conducted was evalu...
A Review on Gongronema latifolium, an Extremely Useful Plant with Great Prospects
Gongronema latifolium is a plant that has a wide range of nutritional and ethnomedical uses in different tropical African communities. Scientific reports on the chemical composition and bioactivity (anti-inflammatory, an...
Phytotherapy of Djallonke Lambs Co-infected with Teladorsagia circumcincta and Trichostrongylus colubriformis (Trichostrongylidae) Using Methanol Extracts of Two Medicinal Plants in Menoua Division, West Region of Cameroon
This study was performed on the West African Dwarf sheep which were experimentally infected with Teladorsagia circumcincta and Trichostromgylus colubriformis. We intended to investigate the anthelmintic potential of two...
Can Leafy Vegetable Source of Omega-3 fatty Acids Ameliorate Acute Intestinal Inflammation Induced in Mice? A Case study of Purslane (Portulaca oleracea)
Aim: To investigate the ameliorative effect of purslane (Portulaca oleracea), an omega-3 rich green leafy vegetable in the treatment therapy of experimental ulcerative colitis in mice. Study Design: In vivo evaluation of...