Anti-Inflammatory Effect of Nano-Silver in Chronic Rhinosinusitis Mouse Model
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 11, Issue 1
Abstract
Objective: Nano-silver has antimicrobial and disinfectant properties with considerable cellular toxicity. The aim of this study was to investigate the effect of nano-silver on chronic rhinosinusitis (CRS) using an eosinophilic CRS mouse model. Methods: The CRS mouse model was established with ovalbumin and Staphylococcus aureus enterotoxin B (SEB). Mice were treated with SEB for 16 weeks and nano-silver was intranasal applied three times a week from weeks 9 through 16. The level of inflammatory cell infiltration and chemical mediators in nasal lavage fluid were determined. Histologic changes were observed using hematoxylin and eosin staining and Periodic acid Schiff (PAS) staining. Results: Intranasal administration of nano-silver resulted in a significant decrease in inflammatory cell infiltration and interferon-gamma levels in nasal lavage fluid. Nano-silver treated mice displayed significantly decreased inflammatory cell infiltration and PAS-positive cells in nasal mucosa. However, interleukin-4 level and epithelial thickness were not influenced. Conclusion: Nano-silver effectively reduced Th1 inflammatory response in the eosinophilic CRS mouse model. Although, nano-silver had immunomodulatory effects, for the clinical usage further studies are needed to prove the pharmacokinetic and pharmacodynamic characteristics of nano-silver. Silver has long been used as a disinfectant and antimicrobial agent. Metallic nanoparticles have been reported to have broad spectrum antibacterial properties [1]. The bioactivity and the properties of nano-sized metal particles are a very interesting area for researchers. Many metallic nanoparticles have been utilized as a special class of chemotherapy and are an emerging medical therapy. However, a high dose of metallic nanoparticles can be potently toxic and cytotoxic to organism they enter [2]. High concentration of nano-silver also has a cytotoxic effect associated with the modulation of chemical mediators in peripheral blood mononuclear cells at appropriate concentrations [3]. With the development of nanotechnology, nano-silver has been applied in various products from home appliances to medical devices [4,5]. Silver nanoparticle possess antibacterial and anti-inflammatory activities, and have been used for medical device to prevent infection [6]. The antibacterial activity of nano-silver is commonly studied and these nanoparticles have been demonstrated capable of restricting the survival of Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes [7,8]. Nano-silver can attenuate Staphylococcus aureus biofilm effect in chronic rhinosinusitis sheep model [9]. The anti-inflammatory effect of nano-silver was demonstrated in a murine model of allergic contact dermatitis and allergic rhinitis [10,11]. Nano-silver suppressed the expression of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 in dermatitis and suppresses IL-4 and IL-10 in allergic rhinitis. However, antimicrobial and anti-inflammatory mechanisms of nanoparticles are still under investigation and the specific immune characteristics of nanoparticles remain unclear. The properties of nanoparticles vary depending on the size uniformity, bioactivity, and purity of particles, and on the target surface molecules [12]. Nano-silver is widely applied in consumer products ranging from home appliances to medical devices [4]. The bioactivity, biological behavior, and potential toxicity of nano-silver are important areas to research to clarify potential medical applications. Because nanosilver has anti-inflammatory action, we presently used a mouse model of allergic chronic rhinosinusitis to evaluate the effect of nano-silver instillation on nasal mucosa inflammation.
Authors and Affiliations
Seung Heon Shin, Mi Kyung Ye, Mi Hyun Che, Dong Won Lee
Keywords
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- EP ID EP587248
- DOI 10.26717/BJSTR.2018.11.002050
- Views 180
- Downloads 0
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