Anti-Nociceptive Effect of Octreotide, a Somatostatin Analogue in Acute Inflammatory Pain in Rats
Journal Title: The 2nd Annual Meeting of International Center for Neuroscience Research - Year 2021, Vol 2, Issue 1
Abstract
Background: Opiates like morphine are used for the treatment of moderate to severe pain. However long-term use often leads to adverse effects like dependence and tolerance. Thus, newer analgesics with lesser side effects need to be identified. Somatostatin is an inhibitory neurotransmitter which is expressed at spinal level where it modulates the transmission of pain. Unfortunately somatostatin has very short half-life. In the present study, the antinociceptive effect of octreotide (a more stable synthetic analog of somatostatin) was evaluated in acute inflammatory pain in rats. Materials and methods: Adult male Sprague-Dawley rats were divided into three equal groups (n=6/group). Acute inflammatory pain was induced by intraplantar administration of carrageenan. Octreotide (3µg) and morphine (10mg/kg) was administered 2 h after the carrageenan in the thigh on the same side. Mechanical allodynia was measured by von Frey filaments at the site of inflammation at 1 and 2 h of drug administration. Paw odema due to carrageenan was evaluated by the plethysmometer at 2 h of drug administration. The rat activity was examined at same time intervals by using activity monitor. Expression of Somatostatin type 2 receptor (SSTR2) was observed at the lumbar region (L4-L5) of the spinal cord. Results: The dose of octreotide was standardized initially by administration of 3µg, 10µg, 30µg &100µg and measuring the corresponding anti-nociceptive effect. The 3µg dose was finally selected due to optimum effect and absence of adverse reactions. Octreotide decreased allodynia compared saline treated control group. However paw odema was not affected by any of the drugs. Importantly nociception returned to baseline values after administration of morphine. Conclusion: The results show that a low dose of octreotide is effective in reducing nociception, compared to higher doses. However, this anti-nociceptive was not as much as morphine which is considered to be the gold standard drug for pain relief. Our finding is supported by other studies which have also observed antinociception in rodents. This information may have clinical relevance.
Authors and Affiliations
Perminder Singh* and S. B. Ray
Keywords
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