ANTI-PROLIFERATIVE AND APOPTOTIC ACTIVITIES OF SYZYGIUM SAMARANGENSE (WAX APPLE) FRUITS EXTRACT AGAINST HUMAN A549 LUNG CANCER CELL LINES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 9
Abstract
Objective: Medicinal plants have been in use from time immemorial and their utility has been expanding step by step in the present world. The intention of the present study was to check the anti-proliferative and apoptotic activity of Syzygium samarangense fruits methanolic extract against of A549 cell lines.Methods: The methanol extract at different concentrations were tested against A549 human lung cancer cell lines for cell viability or cytotoxicity by MTT assay and the hallmark of the apoptosis was analyzed by DNA fragmentation method. The morphological changes resulted due to apoptosis were investigated by Propidium Iodide (PI) staining technique.Results: The results showed that the tested extracts showed strong and decreased cell viability in a concentration-dependent manner. IC50 value represented that the anti-proliferative activity was found with a minimum concentration of 21.86μg/ml. The presence of ladders of DNA fragments in the DNA fragmentation assay indicates a biochemical hallmark of intrinsic apoptotic cell death. Altered cell morphology after treatment with the extract demonstrated that cells experienced apoptosis.Conclusion: The present findings encourage further for the isolation and identifying, of active components present in S. samarangense fruit pulps to understand the mechanism of action in vivo that can make an allowance for developing chemotherapeutic agent against cancer.Â
Authors and Affiliations
Nivetha Thampi, J. Veronica Shalini
Keywords
Related Articles
- EP ID EP578462
- DOI -
- Views 69
- Downloads 0
MESUAFERRIN A-BIOACTIVE FLAVONOID ISOLATED FROM THE BARK OF MESUA FERREA L. AGAINST PHOSPHOLIPASE A2, CYCLOOXYGENASE AND LIPOXYGENASE: AN IN VITRO, IN VIVO AND IN SILICO APPROACH
Objective: The main objective of the present study was to evaluate the anti-inflammatory activity of isolated bioactive flavonoid Mesuaferrin-A from the bark of Mesuaferrea L. by in vitro, in vivo and in silico approach....
A ROLE FOR PHALERIA MACROCARPA (SCHEFF) BOERL. EXTRACTS IN THE MANAGEMENT OF WOMEN’S PATHOLOGICAL CONDITIONS: A RESEARCH REVIEW
Phaleria macrocarpa (Scheff) Boerl is a medicinal plant that originates from West Papua, Indonesia. The fruit of this plant is known to contain numerous different compounds that produce different bioactivities. Many of t...
A NEW STABILITY INDICATING UPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF METOLAZONE AND SPIRONOLACTONE IN BULK AND IN ITS PHARMACEUTICAL FORMULATIONS
Objective: The objective of the work is to develop and validate a new, simple, highly sensitive RP-UPLC method for simultaneous estimation of Metolazone and Spironolactone in bulk and in its dosage forms. Methods: The me...
ASSESSMENT OF ANTIDIABETIC ACTIVITY OF THE TRADITIONAL INDIAN AYURVEDIC FORMULATION BRAHMI GRITHAM IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
Objective: The aim of the present study was to determine the antidiabetic activity of Brahmi gritham a traditional Indian ayurvedic formulation in streptozotocin-induced diabetes in rats.Methods: Diabetes was induced by...
DESIGN AND EVALUATION OF FAST DISSOLVING ORO-DISPERSIBLE FILMS OF METOCLOPRAMIDE HYDROCHLORIDE USING 32 MULTIFACTORIAL DESIGNS
Objective: The objective of the present work was to develop and optimize fast dissolving orodispersible films containing metoclopramide hydrochloride using 32 multifactorial designs.Methods: The films were prepared by so...