ANTIBACTERIAL ACTIVITY OF FRESH WATER CRAB AND SNAIL AND ISOLATION OF ANTIBACTERIAL PEPTIDES FROM HAEMOLYMPH BY SDS – PAGE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 1
Abstract
Objective: The present study was undertaken to characterize antimicrobial molecules from the fresh water snail and crab.Methods: Collection of haemolymph, preparation of extracts, antimicrobial activity, TLC analysis, SDS PAGE analysis.Results: The result of the present investigation reported that the fresh water snail (Pomacea insularium) and crab (Callinectes sapidus) having remarkable antimicrobial activity in methanol, di-ethyl ether and water extracts. Antimicrobial activity was high in di-ethyl ether extracts of the snail against Streptococcus sp. (37.16±0.76 mm) and methanol extract of crab against E. coli (32.16±0.28 mm). The MIC of extracts ranges between 5 µl to 30 µl methanol extract of both snail and crab inhibited the growth of organisms at very low concentration. Biomolecules from the extract was separated by TLC. The molecular mass of the peptide was determined by SDS PAGE. Peptides from snail and crab haemolymph were ranges in 9 to 110 and 40 to 100 kDa respectively.Conclusion: The present findings suggest that fresh water crab and snail having good antimicrobial activity against pathogenic microbes. Therefore they can be used to treat many pathogenic infections.Â
Authors and Affiliations
Packia Lekshmi N C J, Viveka S, Anusha S, Jeeva S, Raja Brindha J, Selva Bharath M
Keywords
Related Articles
- EP ID EP579166
- DOI -
- Views 67
- Downloads 0
MULTIPARTICULATE DRUG DELIVERY SYSTEMS USING NATURAL POLYMERS AS RELEASE RETARDANT MATERIALS
Now-a-days, the emphasis of pharmaceutical researchers is turned towards the development of more efficacious drug delivery systems with already existing molecule. However, in recent applications, Multiparticulate drug de...
INVESTIGATION OF SOME CHEMICAL CONSTITUENTS AND ANTIOXIDANT ACTIVITY OF ASPARAGUS SPERNGERI
Objective: Asparagus sprengeri (A S) is an ornamental plant cultivated in EGYPT. This study was designed to investigate some lipid and flavanoid constituents, in addition to the evaluation of antioxidant activity of diff...
MEROPENEM INDUCED REDUCTION IN SERUM VALPROATE LEVEL- A CASE REPORT
Objective: To report a case of meropenem induced reduction in serum valproate level. Methods: The clinical data of an epileptic patient who experienced a decrease in seizure control due to a drug interaction between valp...
EFFECT OF AMLODIPINE AND ENALAPRIL ON WOUND HEALING IN DIABETIC WISTAR ALBINO RATS
Objective: A number of structural and functional mechanisms have been identified in the pathogenesis of impaired wound healing in diabetes. Diabetes promotes endothelial dysfunction as evidenced by decreased nitric oxide...
IMPACTS OF DIFFERENT CONCENTRATIONS OF AQUEOUS GREEN TEA EXTRACT ADMINISTERED DURING METHOTREXATE TREATMENT ON SOME SELECTED BLOOD INDICES IN RATS
Objective: This study was designed to investigate the impacts of administering of different concentrations of Aqueous Green Tea Extract (AGTE) (0.625, 1.25 or 2.5%) during Methotrexate (MTX) treatment, and continued for...