Antibacterial Activity of Ganoderma Lucidum and Psidium Gujava against the Most Prevalent Endodontic Pathogens: A Preliminary Microbiological Study
Journal Title: Journal of Dental Health and Oral Research - Year 2021, Vol 2, Issue 3
Abstract
Objective: The article aimed to define chemical constituents of Ganoderma Lucidum (GL) and Psidium Gujava (PG) and to compare the preliminary antibacterial activity of GL & PG with Modified Triple Antibiotic Paste (MTAP) and Calcium hydroxide Ca(OH)2. Material and Methods: Gas Chromatography Mass Spectroscopy (GCMS) was used to define the chemical constituents of Ganoderma Lucidum (GL) and Psidium Gujava (PG). The preliminary antimicrobial activity of hot aqueous and ethanolic extract of GL and PG was evaluated by Agar diffusion assay at 75,50,25,10 and 5 µl/ml concentration and diameter of zone of inhibition was measured .The Minimum Inhibitory Concentration (MIC) was measured by serial dilution method at the highest concentration of 100 µg/ml (stock concentration) in DMSO and serially diluted to working concentration ranging from 0.02 µg /ml. One hundred µl isolated strain of P intermedia, F nucleatum was added to each of the 10 serially diluted tubes so that the final volume per tube was 200 µl and incubated at 37°C for 48-72 h. After the incubation period, by visual inspection of the tubes, the MIC values were determined. Minimum Bactericidal Concentration (MBC) was performed by first 3 or 5 tubes which were sensitive to MIC were plated and colony count was taken. Time kill tests were performed at concentrations equal to MIC, twice the MIC and four times the MIC of the extracts, inoculums of size 1.0x105 CFU/ml were added and incubated at 37 degrees and colony count was taken (CFU/ml). Results: Phytochemical extracts responsible for antibacterial activity of GL are flavanoids, flavanones and xanthose and for PG extract Flavanoids, flavanones, and Xanthose. Modified Triple antibiotic paste (MTAP) has the largest zone of inhibition and smallest MIC and MBC concentration. Correlating the results of disc diffusion assay, MIC and MBC both GL and Ca(OH)2 has shown good antibacterial activity against P intermedia as compared to F nucleatum. However at the MIC values, PG is more sensitive to F nucleatum as compared to P intermedia. GL, PG and Ca(OH)2 have shown 50% killing activity after 2 hours and MTAP 100% killing activity. Conclusion: Within the limitation of the study GL and PG can be used as potential herbal intracanal medicaments in the treatment of root canal infections. Further research needs to be carried out to evaluate the antibacterial activity of these novel herbal intracanal medicaments on biofilm models.
Authors and Affiliations
Sheetal Ghivari*, N Meena, Kishore Bhat, Ulka S Idate, Vijay M Kumbar
Keywords
Related Articles
- EP ID EP699023
- DOI http://dx.doi.org/10.46889/JDHOR.2021.2303
- Views 92
- Downloads 0
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