Antibacterial and Antioxidant Activity of Fahrenheitia zeylanica (Thw.) Airy
Journal Title: STAR Journal - Year 2013, Vol 2, Issue 4
Abstract
Fahrenheitia zeylanica (Thw.) Airy is a lofty evergreen tree belonging to the family Euphorbiaceae. The present study was conducted to evaluate antibacterial and antioxidant activity of solvent extracts of leaves of F. zeylanica. The powdered leaf material was sequentially extracted using ethyl acetate, chloroform and methanol based on polarity. Total phenolic and flavonoid contents were estimated by Folin-Ciocalteau and Aluminium chloride colorimetric estimation method respectively. Antibacterial activity of solvent extracts was assessed by agar well diffusion method against a panel of nine bacteria. Antioxidant efficacy of solvent extracts was determined by DPPH free radical scavenging, ABTS radical scavenging and Ferric reducing assay. Methanol and ethyl acetate extracts of bark and chloroform extract of leaf contained high phenolic and flavonoid contents. Solvent extracts of bark were more effective in inhibiting test bacteria than leaf extracts. Staphylococcus aureus and Pseudomonas aeruginosa were inhibited to maximum extent among Gram positive and Gram negative bacteria respectively. Methanol extracts of leaf and bark scavenged DPPH (with IC50 value of 2.02 and 1.17µg/ml respectively) and ABTS (with IC50 value of 20.89 and 3.42µg/ml respectively) radicals to high extent followed by ethyl acetate and chloroform extracts. The reducing ability was recorded highest in methanol extracts followed by ethyl acetate and chloroform extracts. Bark extracts have shown stronger scavenging and reducing power than leaf extracts. A positive correlation was observed between antioxidant activity and total phenolic contents of extracts. The solvent extracts of bark and leaf of F. zeylanica have shown antibacterial and antioxidant activity which may be attributed to the phytochemicals present in them. The plant can be used as a remedy for treatment of infectious diseases and oxidative stress due to free radical formation. Further, separation of bioactive compounds and determination of their biological activities are under progress.
Authors and Affiliations
Rakesh KN| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Syed Junaid| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Dileep N| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Vinayaka KS| Department of Botany, Kumadvathi First Grade College, Shimoga Road, Shikaripura, Karnataka, India, Prashith Kekuda TR| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Raghavendra HL| College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia
Keywords
Related Articles
- EP ID EP9601
- DOI http://dx.doi.org/10.4314/star.v2i4.6
- Views 421
- Downloads 19
Heterosis and Combining Ability for Grain Yield and Yield Component Traits of Maize in Eastern Ethiopia
No Abstract
Acetylcholinesterase Inhibitory Activity of Green Tea Polyphenols
Inhibition of acetylcholinesterase activity is one of the most popular approaches for treatment of neurological disorders such as Alzheimer’s disease and others. In the present study, we evaluated inhibition of acety...
Inhibitory Effect of Cow Urine against Colletotrichum capsici Isolated from Anthracnose of Chilli (Capsicum annuum L.)
Cow urine has got several applications in agriculture. It is shown to possess inhibitory activity against many phytopathogenic fungi and bacteria. Among various diseases of Chilli, anthracnose is the most important dis...
On-farm Phenotypic Characterization of Indigenous Chicken and their Production System in Bench Maji Zone, South Western Ethiopia
Knowledge and understanding of the chicken production system, unique characteristics, opportunities and constraints are important in the design and implementation of indigenous chicken based development programs. The ind...
Effect of Green Prosopis juliflora Pods and Noug Seed (Guizotia obissynica) Cake Supplementation on Digestibility and Performance of Blackhead Ogaden Sheep Fed Hay as a Basal Diet
This study was conducted to evaluate the effect of supplementation of ground immature Prosopis juliflora pods (PJP), noug seed cake (NSC) and their mixtures at different proportions on feed intake, digestibility, and ave...