Antibacterial and Hemagglutinating Activity of the Fruit Pulp of Trilepisium madagascariense DC
Journal Title: Biotechnology Journal International - Year 2017, Vol 18, Issue 2
Abstract
Aim: This study aimed at exploring the hemagglutinating and antibacterial activities of crude lectin from the fruit pulp of Trilepisium madagascariense DC. Methods: Crude lectin was obtained by maceration of the fruit and ammonium sulphate precipitation. Hemagglutinating activity assay was carried out by determining the ability of the crude extract to agglutinate red blood cells, serial dilution of the crude lectin was done followed by addition of erythrocytes. The crude lectin was subjected to evaluation for inhibition of bacterial growth by the agar well diffusion method against fourteen human pathogenic gram-positive and gram-negative bacteria. Results: The extract agglutinated trypsinized and glutaraldehyde-fixed rabbit and human erythrocytes with higher specificity for rabbit erythrocytes and better agglutination with trypsinized erythrocytes. Among the various sugar tested, the hemagglutination was best inhibited by galactose. The crude lectin demonstrated mild spectrum antibacterial activity against four pathogenic gram-positive and gram-negative bacteria. The minimum inhibitory concentration ranged from 1.56 mg/ml to 6.25 mg/ml for Streptococcus faecalis, Bacillus anthracis, Staphylococcus aureus and Pseudomonas fluorescens. Conclusion: The study showed that there is presence of hemagglutinins in the extract and the extract possess antibacterial potential against some pathogenic bacteria tested and can be developed as alternative antibacterial drug that could be employed for the treatment of infectious diseases caused by these pathogens.
Authors and Affiliations
Oludele O. Odekanyin, Temitope T. Aibinu, Olutayo Abioye
Keywords
Related Articles
- EP ID EP315143
- DOI 10.9734/BJI/2017/31313
- Views 131
- Downloads 0
Antibiosis In vitro of Trichoderma Strains Metabolic Extract on Mycelial Growth and Reproductive Capacity of Fusarium oxysporum Isolated from Pepper Plants (Capsicum annuum L.)
Aims: To inhibit the mycelia growth inhibition and reproductive capacity of important phyto-pathogen fungus: Fusarium oxysporum by cell-extracts from submerged cultures of Trichoderma. Study Design: A complete randomized...
Isolation and Characterization of Cellulase Producing Bacteria from the Gut of Termites (Odontotermes and Heterotermes Species)
Aim: To identify, characterize and compare the cellulolytic potentials of strains isolated from the gut of Odontotermes and Heterotermes species. Methodology: Termites were collected, identified, surface sterilized and...
In Vitro Propagation of Alternanthera sessilis L. from Internode Explant
An efficient protocol was developed for micropropagation of Alternanthera sessilis L. from internode explant. Callus was developed from the cut ends and surface of the internode explants when the Murashige and Skoog (MS)...
Identification of Biomarkers of Stress in Meat of Pigs Managed under Different Mixing Treatments
Aims: The objective of this work was to apply the proteomic approach for identification of animal-based stress biomarkers during pig muscle maturation. Study Design: Pigs were subjected to management treatments that may...
Prevalence of Dyslipidemia on the Basis of Biochemistry Tests in the Parakou University Teaching Hospital (Benin)
Aims: Determine the prevalence of dyslipidemia in patients attended in the Parakou University teaching Hospital Biochemistry Laboratory (Republic of Benin). Study Design: Cross-sectional retrospective study. Place and D...