ANTIBIOTICS RESISTANCE PROFILE AND EXTENDED SPECTRUM BETA-LACTAMASES (ESBLS) PRODUCTION BY SALMONELLA SPECIES ISOLATED FROM HIV/AIDS SUBJECTS IN AKWA IBOM STATE, NIGERIA
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2018, Vol 5, Issue 2
Abstract
The present study investigated the antibiotics resistance profile and ESBLs producing strains of Salmonella isolated from HIV/AIDS seropositive subjects in Akwa Ibom State. Stool samples from 300 HIV/AIDS seropositve persons and 300 HIV/AIDS seronegative subjects (control) were cultured for isolation of Salmonella organisms using standard microbiological techniques. Isolates were characterized and identified using conventional tests and Microgen- Gram Negative Identification System (Microgen Bioproducts, USA). Sensitivity testing on the isolates was performed by Kirby-Bauer method while ESLB - production was detected using double disc synergy antibiotics (Hardy diagnostic, USA). Plasmid profiling and plasmid curing were also carried out. A total of 79 and 13 Salmonella isolates were obtained from HIV/AIDS persons and control group. The resistance profile of Salmonella isolates from HIV/AIDS subjects showed 44.3% of the isolates expressed resistance to Ampicillin- Sulbactam, 60.8% to Tetracycline, 46.8% to Ceftaxidime, 53.2% to Ciprofloxacin and 79.8% to Sulfamethoxazole- Trimethoprim. .Isolates from control subjects expressed resistance of 61.5% to Ampicillin- Sulbactam, and Amoxicillin respectively. Isolates from HIV seropositive subjects produced ESBLs activities of 35.4%, against Amoxicillin + Clavulanic acid, Cefotaxime + Clavulanic acid (29.1%) and Cefotaxidime + Clavulanic acid (32.9% ).The organisms produced TEM and CTX- types of ESBLS. Plasmids extracted exhibited heavy molecular weight ranging 23.13kbp and above. Some isolates retained plasmids after curing. Multi-drug resistance by salmonellae in the study area was a repertoire of high molecular weight materials that are plasmid and chromosomal mediated. This calls for enforcement of laboratory examination before antibiotics prescription and administration especially in immunocompromised conditions.
Authors and Affiliations
Dora Imefon Udoh
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