ANTIBIOTICS RESISTANCE PROFILE OF ESCHERICHIA COLI AND ENTEROBACTER AEROGENES ISOLATED FROM WELL WATERS IN AGO-IWOYE, SOUTHWESTERN NIGERIA
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2013, Vol 2, Issue 2
Abstract
Eighty percent of all diseases are attributed to unsafe water with about 11.4 billion people in the world suffering from major related diseases at various times. This study was carried out to investigate the antibiotics resistance profile of Escherichia coli and Enterobacter aerogenes isolated from well waters in Ago-Iwoye, Southwestern Nigeria. Water samples were collected from ten different wells. The multiple tube fermentation technique was employed to enumerate coliforms using MacConkey broth. Nutrient agar and ethylene methylene blue (EMB) agar were used for the enumeration of Escherichia coli and Enterobacter aerogenes. Biochemical characterization was carried out using standard methods. The disk diffusion method was used to determine the antibiotics susceptibility profiles of the bacterial isolates. Results showed that wells contained most probable number (MPN) of bacteria ranging from 43 to 1,100 bacteria per 100 ml. E. coli was present in eight of the wells while E. aerogenes was present in all the wells. Wells B and C had the highest incidence of E. coli with 5.0 x 102 CFU/100ml while wells D and J had no incidence of E. coli. Occurrence of Enterobacter aerogenes was highest in well B (4.5 x102 CFU/100ml), followed by well C (4.0 x102 CFU/100ml) while the lowest occurrence was obtained from well F having a count of 2.0 x 102 CFU/100ml. E. coli was resitant to nitrofurantoin, ampicillin, cephalocidine, sulphafurazole, carbenicillin and sulfamethazole while E. aerogenes was sensitive to colistin, gentamicin and nitrofurantoin but resistant to the remaining antibiotics of the Gram negative disc. For the U4 disc, E. coli was susceptible to colistin sulphate and resistant to all other antibiotics. E. aerogenes was resistant to the entire U4 discs. The presence of E. coli and E. aerogenes suggested faecal pollution, hence the quality of the wells fell strongly below the standard of safe drinking water. Most strains of isolates showed relative resistance to antibiotics investigated in this study and this should be of great concerns to researchers.
Authors and Affiliations
Temitope Kudirat Bello, Bello, Olorunjuwon O. , Egberongbe, H. O. , Azeez, Ikimot A. , Oluwadun, Afolabi
Keywords
Related Articles
- EP ID EP654375
- DOI 10.24297/jab.v2i2.1573
- Views 164
- Downloads 0
Searching of Standard Comet Assay Parameters for Detecting Lymphocyte DNA Damages Using Fourteen Different Test Conditions
The alkaline single cell gel electrophoresis technique (SCGE), also known as the comet assay, is an evaluation method for estimating DNA damage and genotoxicity. This assay is used to measure DNA single-strand breaks, al...
Effect of hens egg shell in breeding substratum on growth meat yield of African giant snail Archachatina marginata (Swaison, 1821)
In order to contribute to growth performances of the African giant snail Archachatina marginata in breeding stock, a study was carried out to determine the effect of hen egg shells on the growth and the meat yield of thi...
Trace metal profile of some fruits in Kokori and Abraka Market, Delta State, Nigeria
An investigation was carried out in Abraka and Kokori communities of Delta State, Nigeria in 2013 to evaluate the trace metal concentration in some fruits: pawpaw, banana, orange and African garden egg commonly grown and...
Genetic Variability of Grain Sorghum (Sorghum bicolour L. Monech) Genotypes under Drought Stress Conditions
Twenty two genotypes of grain sorghum (Sorghum bicolour L. Monech.) were evaluated at College of Agricultural Studies, Sudan University of Science and Technology, Shambat, during two successive summer seasons of 2012 and...
ELEVATION OF β HUMAN CHORIONIC GONADOTROPIN HORMONE AND FIBRINOGEN CONCENTRATION AS AN IMMUNOPROTECTIVE MECHANISM DURING PREGNANCY.
This study was aimed at determining the level of serum β human chorionic gonadotropin hormones and fibrinogen concentration of pregnant women in Benin City. Subjects aged between 18 and 42 years participated in the st...