Antimicrobial and Radical Scavenging Activity of Memecylon malabaricum (C.B. Clarke) Cogn. and Memecylon talboltianum Brandis
Journal Title: STAR Journal - Year 2014, Vol 3, Issue 2
Abstract
The present study was conducted to determine antimicrobial and radical scavenging potential of extract of two species of the genus Memecylon (Melastomataceae) viz., M. malabaricum (C.B. Clarke) Cogn. and M. talboltianum Brandis. The shade dried leaf materials of both Memecylon species were extracted using methanol. Antibacterial activity of leaf extracts was evaluated against five drug resistant uropathogenic bacteria by Agar well diffusion assay. Antifungal activity of leaf extracts was tested on the basis of mycelial growth inhibition of Colletotrichum capsici (isolated from anthracnose of chilli). Radical scavenging activity of extracts was determined by performing DPPH free radical scavenging activity. Total phenolic content of extracts was estimated by Folin-Ciocalteau reagent method. The extracts were subjected to preliminary phytochemical analysis to detect the presence of phytoconstituents. Among extracts, extract of M. malabaricum inhibited all test bacteria and inhibitory potential was marked against Gram positive bacteria than Gram negative bacteria. C. capsici was highly susceptible to extract of M. malabaricum when compared to extract of M. talboltianum. Overall, extract of M. malabaricum displayed marked antimicrobial activity than extract of M. talboltianum. Extract of M. malabaricum scavenged DPPH radicals more efficiently (IC50 6.26µg/ml) when compared to extract of M. talboltianum (IC50 43.80µg/ml). The content of total phenolics was also high in leaf extract of M. malabaricum (112µg GAE/mg) than that of M. talboltianum (28µg GAE/mg). Preliminary phytochemical analysis of leaf extracts revealed the presence of flavonoids, tannins, saponins and glycosides in both extracts. The antimicrobial and radical scavenging activity of leaf extracts could be ascribed to the presence of phytochemicals mainly phenolic compounds. These plants appear to be potential candidates for control of anthracnose disease of chilli and for development of agents active against drug resistant uropathogens and oxidative damage.
Authors and Affiliations
Vivek MN| Post Graduate Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India, Yashoda Kambar| Post Graduate Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India, Prashith Kekuda TR| Post Graduate Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India, Manasa M| Post Graduate Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India, Raghavendra HL| College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia
Keywords
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