Antimicrobial Effect of Copper Oxide Nanoparticles on Some Oral Bacteria and Candida Species
Journal Title: Journal of Dental Biomaterials - Year 2017, Vol 4, Issue 1
Abstract
ABSTRACT Statement of Problem: Acid producing bacteria including Streptococcus mutans and lactobacilli cause tooth demineralization and lead to tooth decay. Also, oral colonization of the species of Candida has been reported in many studies that are resistant to antifungal agents. Objectives: In this study, antibacterial and antifungal effects of nano-CuO were studied against some oral bacteria and yeast fungi. Materials and Methods: The minimum inhibitory concentrations (MICs) of copper oxide nanoparticles (CuO NPs) for oral bacterial and fungal test strains were determined in 96-well microtiter plate technique. The agar diffusion test (ADT) was employed to assess the antifungal properties of nystatin. Results: The MIC50 value of CuO NPs was determined at the range of 1–10 µg/ml for S. mutans, < 1 µg/ml for L. acidophilus, and 10 µg/ml for L. casei. Higher concentrations of CuO NPs (100-1000 µg/ml) were effective on the bacterial cell growth, resulting in 100% reduction in the optical density in TSB medium. The cells of Candida albicans, C. krusei and C. glabrata were treated with CuO NPs and the results showed a decrease in fungal growth at a concentration of 1-1000 µg/ml in TSB medium. The MIC50 value of CuO NPs was determined 1000 µg/ml for three species of Candida. The diameter of growth inhibition zones of 1100 µg/ml nystatin was obtained 15-21 mm for clinical isolates of three species of Candida. Conclusions: With respect to the potential bactericidal activity of CuO NPs on various cariogenic bacteria examined in this study, these NPs could be introduce as a candidate control agent for preventing dental caries or dental infections. In our study, on the other hand, Nano copper oxide had a weak effect on the candida species.
Authors and Affiliations
M Amiria, Z Etemadifarb, A Daneshkazemia
Keywords
Related Articles
- EP ID EP246223
- DOI -
- Views 128
- Downloads 0
Shear Bond Strength of Resin Bonded to Bleached Enamel Using Different Modified 35% Hydrogen Peroxides
Statement of Problem: Bleaching systems with different concentrations and applications are widely used to improve the visual appearance of the teeth, but one of the complications of these materials is reduction of bond s...
Dimensional Stability of Two Polyvinyl Siloxane Impression Materials in Different Time Intervals
Statement of the Problem: Dental prosthesis is usually made indirectly; there- fore dimensional stability of the impression material is very important. Every few years, new impression materials with different manufacture...
Comparison of Antibacterial Effect of Fluoride and Chlorhexidine on Two Cariogenic Bacteria: An in Vitro Study
Statement of problem: Dental plaque is the main source for dental caries and there is no proper vaccine that can affect dental plaques. Objectives: Daily use of an efficient anti-plaque product can be very beneficial in...
Effect of Combined Calcium Hydroxide and Accelerated Portland Cement on Bone Formation and Soft Tissue Healing in Dog Bone Lesions
Statement of Problem: Recent literatures show that accelerated Portland cement (APC) and calcium hydroxide Ca (OH)2 may have the potential to promote the bone regeneration. However, certain clinical studies reveal consis...
Effect of Three Common Desensitizers in Reduction of the DentinHypersensitivity after Periodontal Surgery
Statement of Problem: Dentin hypersensitivity is one of the most common c o m p l a i n t s of patients after periodontal treatments which occur after tissue shrinkage. Objectives: The aim of this study was to determine...