ANTIOXIDANT, ANTIBACTERIAL AND CYTOTOXIC ACTIVITIES OF VARIOUS EXTRACTS OF THYSANOLAENA MAXIMA (ROXB) KUNTZE AVAILABLE IN CHITTAGONG HILL TRACTS OF BANGLADESH
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 7
Abstract
Objective: To evaluate the presence of different phytoconstituents and investigate in vitro bioactivities of petroleum ether, chloroform and methanol extracts of Thysanolaena maxima available in Bangladesh.Methods: Phytochemical screening was conducted using the specific standard procedure. Antioxidant activity of the extracts was evaluated using DPPH radical scavenging assay and reducing power assay. Determination of total phenolic and flavonoid contents was also carried out. Antibacterial and cytotoxic activities were investigated using disc diffusion method and brine shrimp lethality bioassay, respectively.Results: The methanol extract showed highest DPPH radical scavenging activity as well as possessed highest phenolic content (IC50 value for DPPH is 36.94±0.62 μg/ml and total phenolic content is 74.39±2.87 in mg/g, GAE) compared to the petroleum ether and chloroform extracts. On the other hand, chloroform extract possessed maximum flavonoid content (81±7.542 in mg/g, QE) and highest reducing power compare to other extracts. All the extracts showed mild to moderate in vitro antibacterial activity with a zone of inhibition ranging from 7 mm to 16 mm. In brine shrimp lethality bioassay, the LC50 values for petroleum ether, chloroform and methanol extracts were found to be 579.05±78.08 μg/ml, 386.92±80.47 μg/ml and 494.29±104.82 μg/ml, respectively which revealed weak cytotoxic potentials of the extracts compared to the positive control.Conclusion: The results indicated that T. maxima could be a very potent source of natural radical scavenger. Isolation of active compounds from this plant responsible for producing such bioactivities is underway.Keywords: Thysanolaena maxima, DPPH, Total phenolic contents, Total flavonoid contents, Reducing power, Cytotoxicity, Antimicrobial activity
Authors and Affiliations
Nazia Hoque, Md. Hossain Sohrab, Trisha Debnath, Md. Sohel Rana
Keywords
Related Articles
- EP ID EP576231
- DOI -
- Views 59
- Downloads 0
A RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF INDINAVIR IN CAPSULES
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of Indinavir sulphate (400mg...
ESTIMATION OF STUNTED, WASTED, WASTED AND STUNTED CHILDREN AT TOWN DHADGAON, DISTRICT-NANDURBAR OF MAHARASHTRA STATE, INDIA
Objective: The aim of present study was to estimate the percentage of all three classes of malnutrition such as i) Stunted ii) wasted iii) wasted and stunted at study site, so that these findings could help to formulate...
DEVELOPMENT AND VALIDATION OF PATIENT INFORMATION LEAFLET FOR HEART ATTACK PATIENTS
Objective: To prepare a validated heart attack patient information leaflet and to educate and promote the patient knowledge regarding the disease, lifestyle modification, and medication.Methods: The patient information l...
A SYSTEMATIC REVIEW ON INDIAN FLORAL BIODIVERSITY AS EMINENT RESERVES FOR ALTERNATIVE TREATMENT STRATEGY OF DIABETES MELLITUS
Among the most common chronic diseases in the world, Diabetes mellitus (DM) is an extremely studied and widely manifested multi-factorial disease which deliberately requires multi-modal therapeutic strategies [1]. It has...
THE ROLE OF HONEY WITH ROYAL JELLY IN PROTECTING THE GRAAFIAN FOLLICLES FROM THE TOXICITY OF THE ADRIAMYCIN DRUG
Objective: This study aims to investigate the ability of certain mixture of honey with royal jelly (H+R) to prevent the toxic side effects, caused by the Adriamycin drug. One of the most important side effects of this dr...