Antioxidant, Brine Shrimp Lethality and Antimicrobial Activities of Methanol and Ethyl-Acetate Extracts of Citrus macroptera Montr. Fruit Using In vitro Assay Models
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 14
Abstract
Aims: To study and evaluate In vitro antioxidant, brine shrimp lethality and antimicrobial activities of both methanol and ethyl-acetate extracts of Citrus macroptera Montr. Fruit (Family-Rutaceae). Study Design: In vitro antioxidant, brine shrimp lethality and antimicrobial activities. Place and Duration of Study: Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342. The study was carried out from November 2013 to January 2014. Methodology: In vitro antioxidant activities of the extracts were studied using DPPH radical scavenging assay, NO scavenging assay, total phenol, total flavonoid content, total antioxidant capacity, total tannin content, lipid peroxidation by TBA, lipid peroxidation in human erythrocyte cell, reducing power capacity and cupric reducing capacity assays. Lethality bioassay was performed on Artemia salina Leach nauplii. Antimicrobial activity was investigated by disc diffusion technique. Results: Methanol extract showed better activity than ethyl acetate extract in DPPH, NO, lipid peroxidation by TBA, reducing power capacity assay, total phenol, total flavonoid and total antioxidant capacity assays while ethyl –acetate extract showed more potency than methanol extract in total tannin content, cupric reducing capacity and lipid peroxidation in human erythrocyte assays. In brine shrimp bioassay both extracts showed promising lethal activity but methanol extract was found to be more potent than ethyl acetate extract (χ2=39.874, P<0.0001). In disc diffusion technique among six bacterial species, ethyl acetate extract showed broad spectrum antimicrobial activity against two gram positive Bacillus sublitis and Staphylococcus aureus and one gram negative Escherichia coli. Conclusion: The results demonstrate that methanol and ethyl-acetate extracts of C. macroptera fruit can be used as potential antioxidant, cytotoxic and antimicrobial agents. That is why extensive researches are necessary to search for active principles responsible for these activities.
Authors and Affiliations
Nizam Uddin, Md. Rakib Hasan, Md. Monir Hossain, Md. Mahadi Hasan, Aumit Roy, Tufikul Islam, Md. Sazzad Hossain, Abdullah Faruque, Md. Sohel Rana
Keywords
Related Articles
- EP ID EP344668
- DOI 10.9734/BJPR/2014/11235
- Views 106
- Downloads 0
Barriers of Medication Adherence and Health Outcomes
Medication adherence is a worldwide health care problem. Medication adherence refers to whether patients take medication as prescribed by the healthcare provider. Increasing medication adherence may have a great impact o...
Development and Validation of Densitometry TLC Stability Indicating Method for Quantitative Determination of Azelastine Hydrochloride and Emedastine Difumarate in Their Drug Products
Aims: Stability indicating densitometry-TLC assay was established and validated for determination of azelastine hydrochloride (AZT) and emedastine difumarate (ETD) in the presence of their acid and oxidative degradants....
Phytochemical and Biological Evaluations of Methanolic Extract of Amaranthus graecizans subsp. silvestris (Vill.) Brenan
Amaranthus graecizans subsp. silvestris (AMGRS) has traditionally been used a folk medicine in the treatment of inflammation, gonorrhea and piles without scientific evidence. Therefore, methanolic (MeOH) extract of AMGRS...
Dose Response of Viola odorata on Meiotic and Mitotic Chromosomes of Vicia faba
Aims: Viola odorata is a medicinally important plant. It contains bioactive principle viz violin and probably methyl salicylic ester. The present study was designed to analyze the effect of different doses of this medici...
In vivo and in vitro Toxicity Studies of Crude and Partially Purified Leaf Extracts of Jatropha curcas in Wistar Albino Rats
Aims: Jatropha curcas (J. curcas) is a plant that has numerous medicinal and industrial applications. Evaluation of in vitro and in vivo acute/sub chronic hepato-renal toxicity of crude methanol J. curcas leaf extract on...