Antiproliferative effect of aqueous bark extract of Oroxylum indicum L. on Vigna radiata L. (Green gram) seedlings
Journal Title: The Journal of Phytopharmacology - Year 2018, Vol 7, Issue 2
Abstract
The present investigation was carried out to evaluate the antiproliferative activity of aqueous bark extract of Oroxylum indicum L. on Vigna radiata L. (Green gram) seedlings. For different concentrations of plant extract (250, 500, 1000 and 2500 µg/mL) were treated in a 24 well microplate containing the Vigna radiata L. seeds (n=30) for 24, 48 and 72 hours. Colchicine (50 µg/mL) was used as standard drug. Seed germination and seedlings growth were measured. Mitotic index was calculated for the proliferation of cells. The treatment with plant extracts significantly inhibits the germination of seeds, roots and shoots growth, and reduced the mitotic index in meristem ells of Vigna radiata L. at dose and time dependent manner. Qualitative phytochemical screenings of the aqueous stem bark extract of Oroxylum indicum L. revealed the presence of alkaloids, carbohydrates, glycosides, saponins, phytosterols, phenolic compounds and flavonoids. Saponins were found to be in high concentration in the extract.
Authors and Affiliations
Luk Bahadur Chetry, Manuj K Bharali
Keywords
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