APOPTOTIC EFFECT OF GOLD NANOPARTICLES ON HUMAN COLON CANCER CELL LINES CACO-2 BUT NOT ON THE NORMAL EPITHELIAL CELL LINES WISH
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 7
Abstract
Colorectal cancer (CRC) is the third most common cancer worldwide. Consequently, the discovery of new strategies for therapeutic intervention remains a priority. The gold nanoparticles (AuNPs) have been discovered to exert cytotoxicity and induce apoptosis in some cancer cells. The aim of the present study was to investigate the effect of AuNPs on human colon cancer cell lines (Caco-2) and normal human epithelial cell lines (WISH) in vitro. The two cell lines were treated with different concentrations of AuNPs for 5 days and cytotoxicity of AuNPs was assessed. RT-PCR was used to analyze the expression profiles of pro- and anti-apoptotic genes. Tumor necrosis factor alpha (TNF-α) and interlukin-6 (IL-6) levels were measured in the culture supernatant using ELISA. Results revealed that AuNPs has a cytotoxic effect on Caco-2 cell lines at high and low concentrations, but has no cytotoxic effect on WISH cell lines. Upregulation of the pro-apoptotic gene (bax) was induced in AuNPs treated Caco-2 cell lines but not WISH cells. The levels of IL-6 secreted by Caco-2 cell line treated with AuNPs increased gradually up to 96 h then began to decline thereafter. On the other hand, the levels of TNF-α reached its peak after 24h then started to decrease gradually. In conclusion, AuNPs did not cause acute cytotoxicity for normal human epithelial cell lines (WISH), but they have the ability to induce apoptosis in human colon cancer cell lines (Caco-2).
Authors and Affiliations
Soad Nady
Keywords
Related Articles
- EP ID EP617758
- DOI -
- Views 141
- Downloads 0
REMOVAL OF LEAD AND CADMIUM POLLUTION BY COLUMN TECHNOLOGY USING CERATOPHYLLUM DEMERSUM
Environmental exposure to toxic heavy metals is one of the main critical issues on environmental and public health. Heavy metals are common pollutants in aquatic ecosystems, which are particularly susceptible and often f...
THE POTENTIALS OF CHRYSOPHYLUM ALBIDUM PEELS AS NATURAL ADSORBENT
The physico-chemical properties and functional surface group of Chrysophyllum albidum peel was studied using standard method and Fourier Transformation Spectrocopy (FTIR) respectively to access its potentials as a natura...
COMPARATIVE EVALUATION OF THE FLAVONOIDS CONSTITUENTS IN SOME VERBENAEUS SPECIES CULTIVATED IN EGYPT.
Phytochemical screening of Tectona grandis (1), Verbena bipinnatifida (2), Duranta repens (3), Duranta lorentzii (4), Caryopteris incana (5), Gmelina arborea (6), Gmelina hystrix (7) and Verbena hybrida (8) plants showed...
EFFICACY OF CYPERUS ROTUNDUS RHIZOMES-TUBERS EXTRACTS AGAINST PROTOSCOLECES OF ECHINOCOCCUS GRANULOSUS
Background: Cystic echinococcosis is a zoonotic disease with increasing public health concern. Dissemination of protoscoleces –rich fluid during surgery is a major cause of recurrence, so injection of scolicidal agent in...
ANTI OXIDANT ACTIVITY BY DPPH ASSAY FROM CARICA PAPAYA AQUEOUS LEAVES EXTRACT
Nanotechnology is an interdisciplinary approach in biochemical applications and focusing on synthesis of nanoparticles having improved antioxidant properties against the degenerative diseases and cancer. It is assumed th...